|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the C-terminal cytoplasmic part of STIM1|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.
MA1-19451 was used in immunohistochemistry - paraffin section to elucidate how STIM1- and Orai1-mediated calcium oscillations promote melanoma invasion
|Sun J,Lu F,He H,Shen J,Messina J,Mathew R,Wang D,Sarnaik AA,Chang WC,Kim M,Cheng H,Yang S||The Journal of cell biology (207:535)||2014|
STIM1-Ca(2+) signaling is required for the hypertrophic growth of skeletal muscle in mice.
MA1-19451 was used in western blot to study hypertrophic murine skeletal muscle growth and the essential role of STIM1 regulated Ca(2+) signaling
|Li T,Finch EA,Graham V,Zhang ZS,Ding JD,Burch J,Oh-hora M,Rosenberg P||Molecular and cellular biology (32:3009)||2012|
Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and sarcoplasmic reticulum Ca2+ loading in quiescent junctophilin 1 knock-out myotubes.
MA1-19451 was used in western blot to study the of Orai1 pathway in the control of plasmalemmal calcium permeability
|Li H,Ding X,Lopez JR,Takeshima H,Ma J,Allen PD,Eltit JM||The Journal of biological chemistry (285:39171)||2010|