|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunofluorescence (IF)||10-20 ug/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
|Western Blot (WB)||See 42 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunocytochemistry (ICC)||See 15 publications below|
|ELISA (ELISA)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 22 publications below|
|Immunoprecipitation (IP)||See 6 publications below|
|Immunohistochemistry (IHC)||See 7 publications below|
|Immunofluorescence (IF)||See 4 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mink , Rat , Non-human primate , Hamster , Human , Mouse , Rhesus monkey|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full length recombinant GMP-1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This monoclonal antibody can be used to specifically detect the unconjugated (~17 kDa) form of SUMO-1/GMP-1, as well as proteins covalently ligated to GMP-1 (e.g., RanGAP-1). Lysates tested: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells.
Transport of macromolecules between the nucleus and cytoplasm occurs bi-directionally and is mediated by nuclear pore complexes (NPCs). NPCs are large supermolecular structures that span the nuclear envelope. Although ions, metabolites, and small macromolecules can passively diffuse through the NPC, most larger macromolecules such as proteins and ribonucleoproteins (RNPs) must be transported across the NPC by specific signal- and energy-dependent mechanisms. The Ran protein is required for the bi-directional transport of proteins and RNPs across the NPC. Ran is thought to operate in much the same fashion as other Ras-like GTPases acting as a molecular switch by cycling between the GTP bound and GDP bound forms. Nucleotide exchange by Ran is catalyzed by the GTP exchange factor RCC1, while GTP hydrolysis is catalyzed by the GTPase-activating protein RanGAP1. Two other proteins which bind Ran-GTP and possibly affect GTP hydrolysis include RanBP1 and RanBP2 (Nup 358). The mammalian RanGap-1 protein was originally purified from HeLa cells as a 65-70 kDa homodimer. Subsequent studies have demonstrated that RanGAP1 is highly concentrated at the cytoplasmic periphery of the NPC where it associates with RanBP2. This interaction requires the RanGap1 protein to be conjugated to a ~17 kDa ubiquitin-like protein termed GMP-1 (Gap modifying protein 1) or SUMO-1 (small ubiquitin-related modifier). The unmodified 70 kDa form of RanGAP1 is exclusively cytoplasmic, whereas the 90-kDa modified form of RanGap1 associates with the cytoplasmic fibers of the NPC. Covalent modification of target lysines by SUMO-1/GMP-1 modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. As described above, SUMO-1/GMP-1 is a small polypeptide which shares homology with ubiquitin and appears to be involved in protein targeting. In addition to RanGAP-1, at least three other intranuclear proteins are thought to be modified with SUMO-1/GMP-1. Two additional mammalian cDNAs have also been isolated which encode GMP-1-related proteins; however these proteins remain to be characterized in detail. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO-1 and closely related homologs SUMO-2, SUMO-3, and SUMO-4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1; OFC10; PIC1; SENP2; Sentrin; Small ubiquitin-related modifier 1; small ubiquitin-related modifier-1; SMT3; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; SMT3C; SMT3H3; SUMO-1; SUMO-1 related peptidase; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1
Gene Aliases: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SENTRIN; SMT3; SMT3C; SMT3H3; SMTP3; SUMO-1; SUMO1; UBL1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.