Immunofluorescent analysis of SUV39H1 in HeLa cells, fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether, using a SUV39H1 monoclonal antibody (Product # MA1-25505) at a dilution of 20 µg/mL followed by detection using a Goat anti-Mouse IgG, Atto-488 conjugate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fusion protein, MBP-SUV39H1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25505 detects SUV39H1 from mouse and human samples.
MA1-25505 has been successfully used in immunoprecipitation, and Western blot procedures.
The MA1-25505 immunogen is recombinant fusion protein, MBP-SUV39H1.
This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.