|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||SV40 transformed mouse line B4|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
This antibody reacts with the large T and small T-antigen of SV40 under native and denatured conditions. It will also react with the large T-antigen of BKV, human papovavirus.
The simian virus 40 large T antigen plays important role in SV40 transfection and transformation of the cells. It binds to Rb and p53 suppressor proteins leading to the inactivation of suppressor pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunological purification of rat precartilaginous stem cells and construction of the immortalized cell strain.
MA5-12463 was used in immunocytochemistry to isolate rat precartilaginous stem cells and construct an immortalized cell strain
|Zhang S,Chen A,Hu W,Li M,Liao H,Zhu W,Song D,Guo F||Archives of orthopaedic and trauma surgery (128:1339)||2008|