|Immunocytochemistry (ICC)||1-5 ug/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunoprecipitation (IP)||1-5 µg/ml|
|Western Blot (WB)||1-5 µg/ml|
|Tested Species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Full length protein (purified) (SV40).|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.05M sodium phosphate with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen. Recommended positive controls include SV80 or SV-T2 cells.
The SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Simian vacuolating virus 40; Simian virus 40; SV40 T-antigen
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