|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ul|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is THP-1 cells.
Syk, Spleen tyrosine kinase, is expressed in a wide range of hematopoietic and non-hematopoietic cells and plays a key role in adaptive immune system, which is the immunoreceptor (B cell receptor and Fc-receptors) signaling pathways including Fcgamma receptor-mediated phagocytosis. In hematopoietic cells such as B and T lymphocytes, natural killer cells, mast cells, macrophages and platelets, Syk is involved in the proximal signaling downstream of activated immunoreceptors (e.g. BCR, TCR, Fc receptors) containing immunoreceptor tyrosine-based activation motifs (ITAMs) to which it binds using its tandem SH2 domains. Activated and autophosphorylated Syk then phosphorylates its specific substrates including other enzymes and adaptor proteins, orchestrating a complex series of cellular responses such as cell proliferation, differentiation, survival and phagocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.