Invitrogen

SYT11 Polyclonal Antibody

Product Image Gallery

SYT11 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of U2OS cells using SYT11 Polyclonal antibody (Product # PA5-96970) at dilution of 1:100. Blue: DAPI for nuclear staining.

SYT11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver injury using SYT11 Polyclonal antibody (Product # PA5-96970) at a dilution of 1:100 (40x lens).

SYT11 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using SYT11 Polyclonal antibody (Product # PA5-96970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50 - 1:100

Immunofluorescence (IF)

1:50 - 1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:100

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11.

Conjugate

Unconjugated

Form

Liquid

Concentration

4.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808772

Product Specific Information

Immunogen sequence: WSCCHQQAEK KQKNPPYKFI HMLKGISIYP ETLSNKKKII KVRRDKDGPG REGGRRNLLV DAAEAGLLSR DKDPRGPSSG SCIDQLPIKM DYGEELRSPI TSLTPGESKT TSPSSPEEDV MLGSLTFSVD YNFPKKALVV TIQEAHGLPV MDDQTQGSDP YIKM; Positive Samples: U-251MG, Mouse brain, Rat brain; Cellular Location: Cell junction, Cytoplasmic vesicle, Membrane, Single-pass membrane protein, secretory vesicle, synapse, synaptic vesicle membrane

Target Information

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature. [provided by RefSeq, Apr 2010].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.