Reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Choline kinase (CK, ChoK, holine phosphokinase) is an enzyme responsible for catalyzing the first reaction in the choline pathway for phosphatidylcholine biosynthesis. This reaction involves the transfer of a phosphate group from ATP to choline in order to form phosphocholine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bifunctional choline kinase/ethanolamine kinase CKI1; Choline kinase
Gene Aliases: CKI; CKI1; L3130; L9606.8; YLR133W
UniProt ID: (Baker's yeast) P20485
Entrez Gene ID: (Baker's yeast) 850824