Western blot analysis was performed on tissue extract (30 ug lysate) of Mouse Heart. The blot was probed with Sarcalumenin Mouse Monoclonal Antibody (Product# MA3932, 2ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). Two bands at 160 kDa and 54 kDa corresponding to Sarcalumenin and an alternate splice variant, a 54 kDa glycoprotein was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by overnight wet transfer method. The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Dog, Guinea pig, Mouse, Rabbit|
|Published species reactivity||Dog, Rabbit, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rabbit sarcoplasmic reticulum membrane vesicles.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-932 detects sarcalumenin from canine, guinea pig, mouse and rabbit tissues. This antibody specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.
MA3-932 has been successfully used in Western blot. By Western blot, this antibody detects a 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin, respectively, in rabbit skeletal muscle extracts.
The MA3-932 antigen is purified rabbit sarcoplasmic reticulum membrane vesicles.
Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner. The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin.
These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles.
MA3-932 was used in western blot to analyze slow twitch skeletal muscles and the role of the JP45-calsequestrin complex on calcium entry
|Mosca B,Eckhardt J,Bergamelli L,Treves S,Bongianino R,De Negri M,Priori SG,Protasi F,Zorzato F||The Journal of biological chemistry (291:14555)||2016|
|Not Applicable||Not Cited||
Functional characterization of orbicularis oculi and extraocular muscles.
MA3-932 was used in western blot to assess the function and characterization of extraocular muscles and obicularis oculi
|Sekulic-Jablanovic M,Ullrich ND,Goldblum D,Palmowski-Wolfe A,Zorzato F,Treves S||The Journal of general physiology (147:395)||2016|
Characterization of excitation-contraction coupling components in human extraocular muscles.
MA3-932 was used in western blot to study the role of RyR in regulating calcium levels in extraocular muscles.
|Sekulic-Jablanovic M,Palmowski-Wolfe A,Zorzato F,Treves S||The Biochemical journal (466:29)||2015|
Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle.
MA3-932 was used in western blot to study the reduced regucalcin expression observed in diaphragm muscle in muscular dystrophy and the significance for calcium handling
|Doran P,Dowling P,Donoghue P,Buffini M,Ohlendieck K||Biochimica et biophysica acta (1764:773)||2006|
Glycogen- and PP1c-targeting subunit GM is phosphorylated at Ser48 by sarcoplasmic reticulum-bound Ca2+-calmodulin protein kinase in rabbit fast twitch skeletal muscle.
MA3-932 was used in western blot to investigate the role of the SR-bound CaMKII during the regulation of GS activity
|Sacchetto R,Bovo E,Donella-Deana A,Damiani E||The Journal of biological chemistry (280:7147)||2005|
Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle.
MA3-932 was used in western blot to study the reduced expression of calsequestrin in dystrophic mouse skeletal muscle
|Doran P,Dowling P,Lohan J,McDonnell K,Poetsch S,Ohlendieck K||European journal of biochemistry (271:3943)||2004|
Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.
MA3-932 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia
|Glover L,Heffron JJ,Ohlendieck K||Journal of applied physiology (Bethesda, Md. : 1985) (96:11)||2004|
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
MA3-932 was used in western blot to study the expression of Ca(2+) regulatory membrane protein isoforms in skeletal, cardiac and neonatal muscle fibres
|Froemming GR,Murray BE,Harmon S,Pette D,Ohlendieck K||Biochimica et biophysica acta (1466:151)||2000|
Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle.
MA3-932 was used in western blot to study the effects of chronic low frequency stimulation of fast-twitch muscle on the levels of Ca(2+) regulatory membrane proteins
|Ohlendieck K,Frömming GR,Murray BE,Maguire PB,Leisner E,Traub I,Pette D||Pflugers Archiv : European journal of physiology (438:700)||1999|
Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle.
MA3-932 was used in western blot to investigate the protein components of skeletal muscle membranes in canine muscle
|Ohlendieck K,Briggs FN,Lee KF,Wechsler AW,Campbell KP||European journal of biochemistry (202:739)||1991|
Purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum.
MA3-932 was used in western blot to define properties and structural peculiarities of two sarcoplasmic reticulum glycoproteins
|Leberer E,Timms BG,Campbell KP,MacLennan DH||The Journal of biological chemistry (265:10118)||1990|
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.
MA3-932 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy
|Di Blasi C,Blasevich F,Bellafiore E,Mottarelli E,Gibertini S,Zanotti S,Saredi S,Mantegazza R,Morandi L,Mora M||Neuromuscular disorders : NMD (20:326)||2010|
The origin of tubular aggregates in human myopathies.
MA3-932 was used in immunohistochemistry to study the origin and significance of tubular aggregates in human myopathies
|Chevessier F,Bauché-Godard S,Leroy JP,Koenig J,Paturneau-Jouas M,Eymard B,Hantaï D,Verdière-Sahuqué M||The Journal of pathology (207:313)||2005|