Immunofluorescent analysis of Sarcomeric alpha actin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Sarcomeric alpha actin monoclonal antibody (Product # MA1-26928) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).
|Tested species reactivity||Bovine, Fish, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Reptile, Rat, Xenopus|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified rabbit striated muscle actin.|
|Storage buffer||tissue culture supernatant diluted in PBS|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA1-26928 detects alpha sarcomeric Actin from carp, bovine, mouse, guinea pig, human, rabbit, rat, ovine, snake, and Xenopus laevis samples.
MA1-26928 has been successfully used in immunohistochemistry (paraffin) and IF/ICC procedures.
The MA1-26928 immunogen is purified rabbit striated muscle actin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Valvular cardiac myxoma in a dog.
MA1-26928 was used in immunohistochemistry to report on a case of valvular cardiac myxoma in a dog
|Akkoc A,Ozyigit MO,Cangul IT||Journal of veterinary medicine. A, Physiology, pathology, clinical medicine (54:356)||2007|
ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.
MA1-26928 was used in immunohistochemistry to report molecular and clinicopathologic features of urinary bladder inflammatory myofibroblastic tumor
|Debiec-Rychter M,Marynen P,Hagemeijer A,Pauwels P||Genes, chromosomes and cancer (38:187)||2003|