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Immunofluorescent analysis of Sarcomeric alpha actin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Sarcomeric alpha actin monoclonal antibody (Product # MA1-26928) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).
|Tested species reactivity||Bovine, Fish, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Reptile, Rat, Clawed frog|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified rabbit striated muscle actin.|
|Storage buffer||tissue culture supernatant diluted in PBS|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA1-26928 detects alpha sarcomeric Actin from carp, bovine, mouse, guinea pig, human, rabbit, rat, ovine, snake, and Xenopus laevis samples.
MA1-26928 has been successfully used in immunohistochemistry (paraffin) and IF/ICC procedures.
The MA1-26928 immunogen is purified rabbit striated muscle actin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Valvular cardiac myxoma in a dog.
MA1-26928 was used in immunohistochemistry to report on a case of valvular cardiac myxoma in a dog
|Akkoc A,Ozyigit MO,Cangul IT||Journal of veterinary medicine. A, Physiology, pathology, clinical medicine (54:356)||2007|
ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.
MA1-26928 was used in immunohistochemistry to report molecular and clinicopathologic features of urinary bladder inflammatory myofibroblastic tumor
|Debiec-Rychter M,Marynen P,Hagemeijer A,Pauwels P||Genes, chromosomes & cancer (38:187)||2003|
actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; actin, alpha cardiac muscle 1; actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; alpha actin 1; alpha actin 2; alpha-actin cardiac; alpha-actin-1; alpha-actin-2; alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; cardiac actin; cell growth-inhibiting gene 46 protein; nemaline myopathy type 3; skeletal alpha actin; smooth muscle alpha-actin
0610041G09Rik; a-SMA; AA959943; AAT6; ACTA; Acta-2; ACTA1; ACTA2; ACTC; Actc-1; ACTC1; Acts; ACTSA; Actsk-1; ACTVS; alphaSMA; ASD5; ASMA; BOS_10523; BOS_23286; BOS_23966; CFTD; CFTD1; CFTDM; CMD1R; CMH11; GIG46; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA
(Human) P68133, (Bovine) P68138, (Pig) P68137, (Human) P62736, (Rabbit) P62740, (Bovine) P62739, (Human) P04270, (Bovine) Q3ZC07, (Rat) P68136, (Mouse) P68134, (Rat) P62738, (Mouse) P62737, (Mouse) P68033, (Rat) P68035, (Clawed frog) P04751
(Human) 58, (Bovine) 281592, (Pig) 100154254, (Human) 59, (Rabbit) 100009271, (Bovine) 515610, (Human) 70, (Bovine) 533219, (Rat) 29437, (Mouse) 11459, (Rat) 81633, (Mouse) 11475, (Mouse) 11464, (Rat) 29275, (Clawed frog) 379752