Invitrogen

Sarcomeric alpha Actin Monoclonal Antibody (5C5)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Sarcomeric alpha Actin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Sarcomeric alpha actin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Sarcomeric alpha actin monoclonal antibody (Product # MA1-26928) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).

Sarcomeric alpha Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical (Paraffin) analysis of human skeletal muscle tissue using (Product # MA1-26928) Sarcomeric alpha Actin Monoclonal Antibody (5C5).

Sarcomeric alpha Actin Antibody in Immunohistochemistry (IHC) — Figure 1. Immunocytochemistry detection of striated muscle-specific sarcomeric alpha-actin x200. Positive staining of alpha-actin related antigen is a brown particle in the cytoplasm.

Product Details

MA1-26928

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Rabbit

Published species

Dog, Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

5C5

Immunogen

Purified rabbit striated muscle actin.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Storage buffer

ascites

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_795757

Product Specific Information

This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It does not react with smooth muscle tissue.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NF-κB inhibitor on Toll-like receptor 4 signal-induced expression of angiotensinogen and AT1a receptor in neonatal rat left ventricular myocytes.

Experimental and therapeutic medicine

Jiang H,Wang HY,Wang JW,Lou DY,Niu N,Li GH,Qu P

Published figure using Sarcomeric alpha Actin monoclonal antibody (Product # MA1-26928) in Immunohistochemistry

Thu Nov 01 00:00:00 EDT 2018

Valvular cardiac myxoma in a dog.

Journal of veterinary medicine. A, Physiology, pathology, clinical medicine

Akkoc A,Ozyigit MO,Cangul IT

MA1-26928 was used in immunohistochemistry to report on a case of valvular cardiac myxoma in a dog

Sat Sep 01 00:00:00 EDT 2007

ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.

Genes, chromosomes & cancer

Debiec-Rychter M,Marynen P,Hagemeijer A,Pauwels P

MA1-26928 was used in immunohistochemistry to report molecular and clinicopathologic features of urinary bladder inflammatory myofibroblastic tumor

Wed Oct 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: act; actg; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; Alpha-actin-1; Alpha-actin-2; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; beta-actin; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; dfna20; dfna26; MGC75587; nemaline myopathy type 3; unnamed protein product

Gene Aliases: AAT6; ACTA; ACTA1; ACTA2; ACTC; ACTC1; ACTSA; ACTVS; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; GIG46; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM

UniProt ID: (Human) P68133, (Rabbit) P62740, (Human) P62736, (Human) P68032

Entrez Gene ID: (Human) 58, (Rabbit) 100009506, (Rabbit) 100009271, (Human) 59, (Human) 70, (Rabbit) 100340339

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein