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Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

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Sarcomeric alpha Actinin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Sarcomeric alpha Actinin was performed in formalin-fixed, paraffin-embedded human skeletal muscle tissue using Sarcomeric alpha Actinin Monoclonal Antibody (EA-53) (Product # MA1-22863).

Sarcomeric alpha Actinin Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Differentiation of iPSC lines to cardiomyocytes. ( A ) Overview of the protocol used for differentiation of control and HLHS patient specific iPSC to cardiomyocytes; ( B ) Representative immunocytochemical staining of cardiomyocytes derived from control and HLHS patient specific iPSC lines using antibodies raised against alpha actinin and cardiac troponin. One clone from each patient and unaffected control is shown; however similar data were obtained for the second clone. Scale bar = 100 um.

Sarcomeric alpha Actinin Antibody in Immunohistochemistry (IHC) — Figure 4. An internal promoter at the distal I-band explains phenotypic differences between N- an C-terminal ttna mutants. ( A ) RNA-Seq data from 72 hpf zebrafish trunk (left) and E12.5 mouse hearts (right) depicting accumulation of reads within the intron, upstream of the orthologous exons 116 (zebrafish) and 223 (mouse). The Ttn gene is on the negative strand and so reads within the upstream intron are shown to the right of the black bar, which indicates the position of the relevant exon. ( B ) Transcription from an alternative Titin promoter occurs in zebrafish, mouse, and human heart. (left) PCR amplification using a primer in the upstream exon or an internal primer at or near the internal TSS (as determined by 5'-RACE) as forward primer and a shared reverse primer was performed using cDNA from zebrafish, mouse and human heart. For all three species, products of the expected size were found, supporting transcription from an internal promoter. (right) Cartoon representation of PCR amplification scheme, with exon numbering according to zebrafish transcript. The zebrafish 5' UTR is shorter than that of mouse and human. ( C ) (left) H3K4me3 ChIP-Seq data from human fetal leg muscle (GEO Accession GSM1058781), indicating an active promoter overlies exon 240, which is orthologous to exon 116 in zebrafish. The peak at the far right of the figure represents the conventional human TTN promoter. (right) DNAse-Seq data from human fetal heart (GEO Accession GSM665830) indi

Sarcomeric alpha Actinin Antibody in Western Blot (WB) — Figure 10 Immunoblot analysis demonstrates continued dystrophin expression 12 weeks after i.v. injection of scAAV9.U7.ACCA (A) Immunoblots for dystrophin show a sustained increase in dystrophin protein expression (red) normalized to actinin (green) across four different muscles 23 weeks after treatment. Sample lanes marked with a red X were omitted due to technical issues. (B) Immunoblot quantification confirms a significant increase in dystrophin protein expression with increasing dose. Quantification reported as mean +- 95% CI (shaded region), with individual data points representing individual samples. Quantification reported as mean +- SEM, with individual data points representing individual samples. Statistical analysis was performed on pooled data from all skeletal muscles using one-way ANOVA with a post hoc test for linear trend from left to right; ****p < 0.0001 for trend. Bottom panel displays quantification results broken down by individual muscles.

Product Details

MA1-22863

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:30	-
Immunohistochemistry - Free Floating (IHC (Free))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 5 publications 5 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rabbit, Zebrafish
Published species	Human, Mouse, Pig, Rat, Zebrafish
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	EA-53
Immunogen	Purified rabbit skeletal alpha-actinin.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	Ascites
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_557426

Product Specific Information

MA1-22863 detects sarcomeric alpha Actinin in bovine, feline, chicken, fish, goat, hamster, lizard, mouse porcine, rabbit, rat, ovine, snake, and Xenopus laevis samples.

Target Information

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells.

eLife

Ng WH,Johnston EK,Tan JJ,Bliley JM,Feinberg AW,Stolz DB,Sun M,Wijesekara P,Hawkins F,Kotton DN,Ren X

MA1-22863 was used in Immunocytochemistry to report a novel stepwise strategy for directing the simultaneous induction of both mesoderm-derived cardiac and endoderm-derived lung epithelial lineages within a single differentiation of human-induced pluripotent stem cells (hiPSCs) via temporal specific tuning of WNT and nodal signaling in the absence of exogenous growth factors.

Wed Jan 12 00:00:00 EST 2022

Pre-clinical dose-escalation studies establish a therapeutic range for U7snRNA-mediated DMD exon 2 skipping.

Molecular therapy. Methods & clinical development

Simmons TR,Vetter TA,Huang N,Vulin-Chaffiol A,Wein N,Flanigan KM

MA1-22863 was used in Western Blot to develop an adeno-associated virus (AAV)-based U7snRNA exon-skipping approach directed toward exon 2, duplications of which represent 10% of all DMD duplication mutations.

Fri Jun 11 00:00:00 EDT 2021

Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates.

Scientific reports

Jensen JH,Cakal SD,Li J,Pless CJ,Radeke C,Jepsen ML,Jensen TE,Dufva M,Lind JU

MA1-22863 was used in Immunocytochemistry-immunoflourescence to show that C2C12 myoblasts spontaneously organize into highly aligned myotube tissues on the mm to cm scale, when cultured on sufficiently soft yet fully isotropic gelatin hydrogel substrates.

Thu Aug 06 00:00:00 EDT 2020

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Cells

Filippo Buono M,von Boehmer L,Strang J,Hoerstrup SP,Emmert MY,Nugraha B

MA1-22863 was used in Immunocytochemistry to demonstrate the generation of cardiac organoids using a triculture approach of human induced pluripotent stem-cell-derived cardiomyocytes (hiPS-CMs)- from healthy subjects and cardiomyopathy patients-human cardiac microvascular endothelial cells (HCMECs) and human cardiac fibroblasts (HCFs).

Mon Jul 20 00:00:00 EDT 2020

On-site fabrication of Bi-layered adhesive mesenchymal stromal cell-dressings for the treatment of heart failure.

Biomaterials

Kobayashi K,Ichihara Y,Sato N,Umeda N,Fields L,Fukumitsu M,Tago Y,Ito T,Kainuma S,Podaru M,Lewis-McDougall F,Yamahara K,Uppal R,Suzuki K

MA1-22863 was used in Immunohistochemistry to address the issue of poor donor cell engraftment following current mesenchymal stromal/stem cell delivery methods.

Mon Jul 01 00:00:00 EDT 2019

Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.

Human molecular genetics

Yang C,Xu Y,Yu M,Lee D,Alharti S,Hellen N,Ahmad Shaik N,Banaganapalli B,Sheikh Ali Mohamoud H,Elango R,Przyborski S,Tenin G,Williams S,O'Sullivan J,Al-Radi OO,Atta J,Harding SE,Keavney B,Lako M,Armstrong L

Published figure using alpha Sarcomeric Actinin monoclonal antibody (Product # MA1-22863) in Immunocytochemistry

Tue Aug 15 00:00:00 EDT 2017

An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish.

eLife

Zou J,Tran D,Baalbaki M,Tang LF,Poon A,Pelonero A,Titus EW,Yuan C,Shi C,Patchava S,Halper E,Garg J,Movsesyan I,Yin C,Wu R,Wilsbacher LD,Liu J,Hager RL,Coughlin SR,Jinek M,Pullinger CR,Kane JP,Hart DO,Kwok PY,Deo RC

MA1-22863 was used in immunohistochemistry to characterize zebrafish with Titin truncations

Fri Oct 16 00:00:00 EDT 2015

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.

Nature communications

Sharma P,Abbasi C,Lazic S,Teng ACT,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO

MA1-22863 was used in immunocytochemistry to investigate the impact of Tmem65 on heart development

Fri Sep 25 00:00:00 EDT 2015

Amniotic fluid stem cells morph into a cardiovascular lineage: analysis of a chemically induced cardiac and vascular commitment.

Drug design, development and therapy

Maioli M,Contini G,Santaniello S,Bandiera P,Pigliaru G,Sanna R,Rinaldi S,Delitala AP,Montella A,Bagella L,Ventura C

MA1-22863 was used in immunocytochemistry to study the ability of a mixture of hyaluronic, butyric and retinoic acids to promote the differentiation of amniotic stem cells towards a cardiovascular lineage

Mon May 05 00:00:00 EDT 2014

Dystrophin-deficient cardiomyocytes derived from human urine: new biologic reagents for drug discovery.

Stem cell research

Guan X,Mack DL,Moreno CM,Strande JL,Mathieu J,Shi Y,Markert CD,Wang Z,Liu G,Lawlor MW,Moorefield EC,Jones TN,Fugate JA,Furth ME,Murry CE,Ruohola-Baker H,Zhang Y,Santana LF,Childers MK

MA1-22863 was used in immunohistochemistry to study the generation of cardiomyocytes from human urine-derived stem cells obtained from a patient with Duchenne muscular dystrophy

Sat Mar 01 00:00:00 EST 2014

Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes.

Science translational medicine

Shapiro SD,Ranjan AK,Kawase Y,Cheng RK,Kara RJ,Bhattacharya R,Guzman-Martinez G,Sanz J,Garcia MJ,Chaudhry HW

MA1-22863 was used in immunocytochemistry and immunohistochemistry to study the role of adult cardiomyocyte cytokinesis in the mechanism by which adenoviral delivery of cyclin A2 to infarcted porcine heart promotes regeneration

Wed Feb 19 00:00:00 EST 2014

Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; Non-muscle alpha-actinin-1

Gene Aliases: 3110023F10Rik; ACTN1; Actn1a; BDPLT15

UniProt ID: (Human) P12814, (Mouse) Q7TPR4

Entrez Gene ID: (Human) 87, (Rabbit) 100341378, (Mouse) 109711, (Zebrafish) 560400

Function(s)

platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription cortical cytoskeleton organization

Process(es)

double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding actin binding protein domain specific binding metal ion binding

Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

Product Details

MA1-22863

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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