Immunofluorescent analysis of Scavenger Receptor SR-BI + SR-BII in Hela cells using a Scavenger Receptor SR-BI + SR-BII polyclonal antibody (Product # PA1-32415) and detected with a Dylight 488 labeled secondary antibody (Green). Nuclei were visualized using Hoechst 33258 counterstain (Blue).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII was expressed as two tandem copies in bacteria using the pET system.|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32415 detects SR-BI + SR-BII from mouse samples.
PA1-32415 has been successfully used in immunofluorescence, immunohistochemistry, immunoprecipitation, Western blot applications.
The PA1-32415 immunogen is: Synthetic peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII was expressed as two tandem copies in bacteria using the pET system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.