Whole cell lysates of serum starved HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), Jurkat (5) U937 (6), K562 (7) and THP (8) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-Scramblase 1 antibody (Catalog no. 44318M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using an ECL detection method (exposure time: 30 seconds).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Peptide conjugated to hemocyanin.|
|Storage buffer||PBS, pH 7.3, with PEG, sucrose|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g., epidermal growth factor, EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for Src kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.