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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
ELISA (ELISA) |
1:5,000-1:20,000 | - |
Immunoprecipitation (IP) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
2.3 mg/mL |
Purification |
Protein A |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1. A BLAST analysis was used to suggest cross-reactivity with SPS2 from human sources based on an 84% homology with the immunizing sequence. Cross-reactivity with SPS2 from other sources has not been determined.
This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: clone 1000; selenide; Selenide, water dikinase 2; Selenium donor protein 2; Selenophosphate synthase 2; the SelD gene product
Gene Aliases: Sephs2; Sps2; Ysg3
UniProt ID: (Mouse) P97364
Entrez Gene ID: (Mouse) 20768
Molecular Function:
transferase
metabolite interconversion enzyme
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