|Tested species reactivity||Sheep|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2,000-1:4,000|
|Western Blot (WB)||1:2,000-1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Anti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Airway bacteria drive a progressive COPD-like phenotype in mice with polymeric immunoglobulin receptor deficiency.
61-8620 was used in western blot to characterize mice with polymeric immunoglobulin receptor deficiency and airway bacteria that drive a progressive COPD-like phenotype
|Richmond BW,Brucker RM,Han W,Du RH,Zhang Y,Cheng DS,Gleaves L,Abdolrasulnia R,Polosukhina D,Clark PE,Bordenstein SR,Blackwell TS,Polosukhin VV||Nature communications (7:null)||2016|
Epidermal growth factor receptor-dependent and -independent pathways in hydrogen peroxide-induced mitogen-activated protein kinase activation in cardiomyocytes and heart fibroblasts.
61-8620 was used in western blot to investigate the role of the epidermal growth factor receptor in H2O2-induced MAPK activation using cardiac cells
|Purdom S,Chen QM||The Journal of pharmacology and experimental therapeutics (312:1179)||2005|