|Tested species reactivity||Sheep|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.
Anti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Hormone-sensitive lipase serine phosphorylation and glycerol exchange across skeletal muscle in lean and obese subjects: effect of beta-adrenergic stimulation.
81-8620 was used in western blot to test if a blunted fasting or beta-adrenergically mediated lipolysis contributes to increased intramuscular triacylglycerol storage in obesity
|Jocken JW,Roepstorff C,Goossens GH,van der Baan P,van Baak M,Saris WH,Kiens B,Blaak EE||Diabetes (57:1834)||2008|
|Not Applicable||Not Cited||
Sex differences in hormone-sensitive lipase expression, activity, and phosphorylation in skeletal muscle at rest and during exercise.
81-8620 was used in western blot to elucidate the sex-specific usage of intramuscular triacylglycerol during exercise
|Roepstorff C,Donsmark M,Thiele M,Vistisen B,Stewart G,Vissing K,Schjerling P,Hardie DG,Galbo H,Kiens B||American journal of physiology. Endocrinology and metabolism (291:E1106)||2006|
Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla.
81-8620 was used in immunohistochemistry - paraffin section to investigate the effect of lipopolysaccharide treatment on V2 receptors and aquaporin-2 expression in the kidney
|Grinevich V,Knepper MA,Verbalis J,Reyes I,Aguilera G||Kidney international (65:54)||2004|