|Tested species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Bacterially expressed Sigma 54 from Balb/c Sp2/0 cells, purified over a heparin column|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-5877 detects Sigma 54 in bacterial samples.
MA1-5877 has been successfully used in Western blot and ELISA procedures. MA1-5877 binds specifically in ELISA to monomeric tetrameric Sigma 54 but it does not bind to RAT protein or AgrA protein.
The MA1-5877 immunogen is bacterially expressed Sigma 54 and purified over a heparin column.
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein glnG(ntrC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.