Immunofluorescent analysis of Sin3A (green) showing staining in the nucleus of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sin3A polyclonal antibody (Product # PA1-870) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-870 detects mSin3A from human and mouse tissues and cells.
PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.
PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A. This immunizing peptide (Cat. # PEP-108) is available for use in neutralization and control experiments.
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.
PA1-870 was used in western blot to study the modulation of MCL1 alternative splicing by an RNA-dependent mechanism involving dynamic histone acetylation
|Khan DH,Gonzalez C,Cooper C,Sun JM,Chen HY,Healy S,Xu W,Smith KT,Workman JL,Leygue E,Davie JR||Nucleic acids research (42:1656)||2014|
Protein kinase CK2 regulates the dimerization of histone deacetylase 1 (HDAC1) and HDAC2 during mitosis.
PA1-870 was used in western blot to study the modulation of the dimerization of HDAC-1 and -2 by CK2-mediated phosphorylation during mitosis
|Khan DH,He S,Yu J,Winter S,Cao W,Seiser C,Davie JR||The Journal of biological chemistry (288:16518)||2013|
Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin.
PA1-870 was used in immunocytochemistry to study the mitotic dynamics of HDAC-1 and -2 and their association with F-actin
|He S,Khan DH,Winter S,Seiser C,Davie JR||Journal of cellular physiology (228:1525)||2013|