Invitrogen

Skeletal Muscle Actin Monoclonal Antibody (5C5.F8.C7 (alpha-Sr-1))

Product Image Gallery

Skeletal Muscle Actin Antibody — Figure 2. Subcellular localization of endogenous Actalpha, Actbeta, and Actgamma mRNAs in cultured motoneurons. (A) In situ hybridization signals for Actalpha (A), Actbeta (B), and Actgamma transcripts (C) were detected in soma, axons, and axonal growth cones of cultured motoneurons. Cells were immunostained against Tau to determine cell boundaries. (D-F) Quantification of FISH signals shows that Actbeta and Actgamma are detectable at similar levels in the soma, but Actbeta is more abundant in the axon and axonal growth cone (***, P < 0.0001 by one-way ANOVA with Dunn's post-test for n = 6). Shown are mean +- SEM. (G) In triplex assays, cells were incubated simultaneously with probes detecting transcripts of Actalpha (white), Actbeta (green), and Actgamma (blue). Differential localization of actin mRNAs in soma, axons, and growth cones indicates that these mRNAs are present in different RNP granules. White circles and arrowheads in A and G indicate Actalpha mRNA punctae. Bars, 10 um.

Skeletal Muscle Actin Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Actin Skeletal Muscle was done on 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Actin Skeletal Muscle (5C5.F8.C7 (alpha-Sr-1)) Mouse Monoclonal Antibody (Product # MA5-12542) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Skeletal Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Actin Skeletal Muscle showing staining in the cytoplasm of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Actin Skeletal Muscle Mouse Monoclonal Antibody (Product # MA5-12542) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Skeletal Muscle Actin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human skeletal muscle stained with Skeletal Muscle Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of skeletal muscle cells.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Published Applications

Immunofluorescence (IF)

See 1 publication below

Western Blot (WB)

See 1 publication below

Immunohistochemistry (IHC)

See 4 publications below

Product Specifications

Species Reactivity

Amphibian, Bovine, Guinea pig, Human, Mouse, Sheep, Rabbit, Rat

Published species

Human, Rabbit

Host / Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

5C5.F8.C7 (alpha-Sr-1)

Immunogen

N-terminal decapeptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10984949

Product Specific Information

MA5-12542 targets Actin Skeletal Muscle in IF and IHC (P) applications and shows reactivity with Amphibian, Bovine, Guinea Pig, Human, Ovine, Rabbit, and Rat samples.

The MA5-12542 immunogen is n-terminal decapeptide.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actin; actin alpha 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; alpha actin; alpha actin 2; alpha skeletal muscle; alpha skeletal muscle actin; Alpha-actin-1; Alpha-actin-2; alpha-cardiac actin; alpha-smooth muscle actin; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; nemaline myopathy type 3; smooth muscle alpha-actin

Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACTA; Acta-2; ACTA1; ACTA2; Acts; ACTSA; Actsk-1; ACTVS; alphaSMA; ASMA; BOS_23286; BOS_23966; CFTD; CFTD1; CFTDM; GIG46; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA

UniProt ID: (Human) P68133, (Bovine) P68138, (Rabbit) P62740, (Bovine) P62739, (Human) P62736, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P03996

Entrez Gene ID: (Human) 58, (Bovine) 281592, (Sheep) 101107662, (Rabbit) 100009271, (Bovine) 515610, (Human) 59, (Sheep) 101121051, (Guinea pig) 100716933, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Guinea pig) 100722917, (Rabbit) 100009506