|Tested species reactivity||Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Rabbit, Fruit fly, Hamster, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG3, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90490 detects Smith Antigen from rat, rabbit, human, and pig samples.
MA1-90490 has been successfully used in immunomicroscopy, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin tissue), Western blot applications.
Positive control: LS174T cells, Tonsil.
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodi
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.
MA1-90490 was used in immunoprecipitation to study the role of a mutation of a U2 snRNA gene in global disruption of alternative splicing and neurodegeneration
|Jia Y,Mu JC,Ackerman SL||Cell (148:296)||2012|
PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.
MA1-90490 was used in immunoprecipitation to investigate the associates of PRPF mutations with defects in pre-mRNA splicing in patients with retinitis pigmentosa
|Tanackovic G,Ransijn A,Thibault P,Abou Elela S,Klinck R,Berson EL,Chabot B,Rivolta C||Human molecular genetics (20:2116)||2011|
Combination of SMN trans-splicing and a neurotrophic factor increases the life span and body mass in a severe model of spinal muscular atrophy.
MA1-90490 was used in immunoprecipitation to investigate the effectiveness of SMN1 gene therapy in the treatment of spinal muscular atrophy in a mouse model
|Shababi M,Glascock J,Lorson CL||Human gene therapy (22:135)||2011|
Functional association of the Microprocessor complex with the spliceosome.
MA1-90490 was used in immunoprecipitation to investigate the molecular basis for intronic miRNA processing
|Kataoka N,Fujita M,Ohno M||Molecular and cellular biology (29:3243)||2009|
Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing.
MA1-90490 was used in immunoprecipitation to study the restoration of survival motor neuron function by a redirection of SMN2 pre-mRNA splicing
|Coady TH,Shababi M,Tullis GE,Lorson CL||Molecular therapy : the journal of the American Society of Gene Therapy (15:1471)||2007|
Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly.
MA1-90490 was used in immunoprecipitation to investigate the role of gemin8 in the survival motor neuron complex and its function in small nuclear ribonucleoprotein assembly
|Carissimi C,Saieva L,Baccon J,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L||The Journal of biological chemistry (281:8126)||2006|
Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
MA1-90490 was used in immunoprecipitation and western blot to study the coupled nuclear import of U snRNPs and the survival of motor neurons protein
|Narayanan U,Achsel T,Lührmann R,Matera AG||Molecular cell (16:223)||2004|
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
MA1-90490 was used in immunoprecipitation to study the mechanism for the inhibitory effect of pICln on snRNP biogenesis
|Pu WT,Krapivinsky GB,Krapivinsky L,Clapham DE||Molecular and cellular biology (19:4113)||1999|
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.
MA1-90490 was used in immunocytochemistry to evaluate the utility of fully grown germinal vesicle stage oocyte cytoplasm in somatic cell nuclear transfer experiments
|Fulka H,Novakova Z,Mosko T,Fulka J||Histochemistry and cell biology (132:457)||2009|
|Not Applicable||Not Cited||
Conserved patterns of nuclear compartmentalization are not observed in the chordate Oikopleura.
MA1-90490 was used in immunocytochemistry to study the characteristics of nuclear compartmentalization in the chordate Oikopleura
|Spada F,Koch J,Sadoni N,Mitchell N,Ganot P,De Boni U,Zink D,Thompson EM||Biology of the cell / under the auspices of the European Cell Biology Organization (99:273)||2007|
|Fruit fly||Not Cited||
Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.
MA1-90490 was used in immunocytochemistry to study the essential role of protein arginine methyltransferase in protein localization in germ cells
|Anne J,Ollo R,Ephrussi A,Mechler BM||Development (Cambridge, England) (134:137)||2007|
Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins.
MA1-90490 was used in immunocytochemistry and western blot to study nuclear substructure reorganization during late-stage erythropoiesis
|Krauss SW,Lo AJ,Short SA,Koury MJ,Mohandas N,Chasis JA||Blood (106:2200)||2005|
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.
MA1-90490 was used in immunocytochemistry to study the role of Tim50a in snRNP biogenesis.
|Xu H,Somers ZB,Robinson ML,Hebert MD||BMC cell biology (6:null)||2005|
A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules.
MA1-90490 was used in immunocytochemistry to study the role of stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules
|Metz A,Soret J,Vourc'h C,Tazi J,Jolly C||Journal of cell science (117:4551)||2004|
ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia.
MA1-90490 was used in immunocytochemistry to study the disruption of Cajal body components ELL and EAF1 in MLL-ELL leukemia
|Polak PE,Simone F,Kaberlein JJ,Luo RT,Thirman MJ||Molecular biology of the cell (14:1517)||2003|
Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.
MA1-90490 was used in immunohistochemistry to investigate the role of mutations in splicing factor PRPF3 in retinal degeneration of photoreceptor cells
|Comitato A,Spampanato C,Chakarova C,Sanges D,Bhattacharya SS,Marigo V||Human molecular genetics (16:1699)||2007|
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
MA1-90490 was used in immunohistochemistry to study the role of the myosin16b COOH-tail region in localization to the nucleus and S-phase progression
|Cameron RS,Liu C,Mixon AS,Pihkala JP,Rahn RJ,Cameron PL||Cell motility and the cytoskeleton (64:19)||2007|
Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos.
MA1-90490 was used in immunohistochemistry to study the role of protein kinase C delta and epsilon subcellular localization on transcriptional and post-transcriptional processes in mouse preimplantation development
|Dehghani H,Reith C,Hahnel AC||Reproduction (Cambridge, England) (130:453)||2005|
ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies.
MA1-90490 was used in immunohistochemistry to investigate the role of ZPR1 for the motor neurons (SMN) survival and the localization of the survival motor neurons (SMN) protein to Cajal bodies
|Gangwani L,Flavell RA,Davis RJ||Molecular and cellular biology (25:2744)||2005|