|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1-3µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 2–10 of human Smooth Muscle Actin|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with bovine, canine, rabbit, rat and porcine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Smooth muscle is found in the walls of blood vessels, lymphatic vessels, and a number of other tissues, where it provides mechanical support, and is a major component of the contractile apparatus. One of the primary components of smooth muscle is actin, which has six known isotypes in mammals. Smooth muscle actin exists in alpha-and gamma-actin isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAT6; actin; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; ACTSA; alpha 2 actin; Alpha-actin-2; alpha-cardiac actin; alpha-sma; aortic smooth muscle; Cell growth-inhibiting gene 46 protein; GIG46; growth-inhibiting gene 46; MYMY5; smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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