Immunohistochemical analysis of smooth muscle alpha-actin paraffin embedded Human Lung tissue (top) and Mouse Lung tissue (bottom). Trypsin-mediated antigen retrieval was performed, followed by application of smooth muscle alpha-actin antibody (Product # PA5-18292) (2.5 µg / ml) and HRP-staining. Bar in right lower corner represents 50um. Data provided by Dr L. Marsh, Ludwig Boltzmann Institute for Lung Vascular Research, Graz, Austria.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (EEEDSTALVC) corresponding to the N-terminus amino acids of ACTA2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody is predicted to react with canine based on sequence homology.
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Combination Growth Factor Therapy via Electrostatically Assembled Wound Dressings Improves Diabetic Ulcer Healing In Vivo.
PA5-18292 was used in immunohistochemistry to calculate an improvement in diabetic ulcer healing in vivo by combination growth factor therapy via electrostatically assembled wound dressings
|Almquist BD,Castleberry SA,Sun JB,Lu AY,Hammond PT||Advanced healthcare materials (null:null)||2015|