|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: D(90) L N D K I R D Y T P Q P(102)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-926 detects rat sodium/calcium-potassium exchanger (NCKX2) overexpressed in HEK cells transfected with the rat gene.
PA1-926 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 78 kDa protein representing recombinant rat NCKX2. Immunohistochemical staining of rat NCKX2 in HEK cells transfected with the rat gene with PA1-926 results in staining of the plasma membrane.
PA1-926 immunizing peptide corresponds to amino acid residues 90-102 from rat NCKX2. This sequence is predicted to be on the extracellular side of the plasma membrane. PA1-926 immunizing peptide (Cat. # PEP-031) is available for use in neutralization and control experiments.
Sodium/calcium-potassium exchanger proteins (NCKX1) have been reported to occur in retinal photoreceptors and in brain (NCKX2). Unlike the abundant and widely expressed sodium/calcium exchanger which catalyzes the exchange of 3 sodium ions for 1 calcium ion to modulate intracellular calcium levels, NCKX isoforms exchange 4 sodium ions for 1 calcium and 1 potassium ion. The sodium/calcium-potassium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium in a potassium-dependent mechanism against its electrochemical gradient. It is hypothesized that NCKX2 plays a significant role in calcium homeostasis and the regulation of neuronal function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distribution of K+-dependent Na+/Ca2+ exchangers in the rat supraoptic magnocellular neuron is polarized to axon terminals.
PA1-926 was used in immunohistochemistry to demonstrate the polarized distribution of sodium/calcium exchanger in the rat supraoptic magnocellular neuron.
|Kim MH,Lee SH,Park KH,Ho WK,Lee SH||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:11673)||2003|