Immunofluorescent analysis of sodium/potassium ATPase alpha-1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a sodium/potassium ATPase alpha-1 monoclonal antibody (Product # MA3-929) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:500 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin (Product # 21836) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Sheep, Pig, Rat|
|Published species reactivity||Dog, Yeast, Rat, Non-human primate, Sheep, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Lamb kidney alpha 1 sodium/potassium ATPase.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/20 - 1/100|
|Immunocytochemistry (ICC)||1/50 - 1/200|
|Immunofluorescence (IF)||1/50 - 1/200|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100 - 1/2000|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-929 detects sodium/potassium ATPase alpha 1 in sheep, canine, rat, mouse, human and porcine tissues. This antibody does not detect the alpha 1 isoform in Xenopus and chicken. MA3-929 does not detect the alpha 2 or alpha 3 isoform in rat.
MA3-929 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract. Immunohistochemical staining of sodium/potassium ATPase alpha 1 with MA3-929 yields a pattern consistent with plasma membrane localization.
The MA3-929 antigen is sheep kidney alpha 1 sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FXYD2, a ¿ subunit of Na¿, K¿-ATPase, maintains persistent mechanical allodynia induced by inflammation.
MA3-929 was used in immunoprecipitation and western blot to determine a role for FXYD2 in neurons using knock out mice
|Wang F,Cai B,Li KC,Hu XY,Lu YJ,Wang Q,Bao L,Zhang X||Cell research (25:318)||2015|
Large-conductance Ca²¿-activated potassium channel in mitochondria of endothelial EA.hy926 cells.
MA3-929 was used in western blot to study the presence and functional characteristics of a large-conductance Ca(2+)-activated K(+) channel in EA.hy926 endothelial cell inner mitochondrial membranes
|Bednarczyk P,Koziel A,Jarmuszkiewicz W,Szewczyk A||American journal of physiology. Heart and circulatory physiology (304:H1415)||2013|
Isoforms of protein 4.1 are differentially distributed in heart muscle cells: relation of 4.1R and 4.1G to components of the Ca2+ homeostasis system.
MA3-929 was used in western blot to study the expression and localization of protein4.1 isoforms in cardiac muscle and the significance for cardiomyocyte Ca(2+) metabolism
|Pinder JC,Taylor-Harris PM,Bennett PM,Carter E,Hayes NV,King MD,Holt MR,Maggs AM,Gascard P,Baines AJ||Experimental cell research (318:1467)||2012|
A dual mechanism for I(Ks) current reduction by the pathogenic mutation KCNQ1-S277L.
MA3-929 was used in western blot to investigate the role of a deleterious mutation of KCNQ1-S277L in hereditary long QT syndrome
|Chen J,Weber M,Um SY,Walsh CA,Tang Y,McDonald TV||Pacing and clinical electrophysiology : PACE (34:1652)||2011|
|Non-human primate||10 nM||
Mechanism of anion selectivity and stoichiometry of the Na+/I- symporter (NIS).
MA3-929 was used in western blot to study the structural and mechanistic features of sodium iodine symporter
|Paroder-Belenitsky M,Maestas MJ,Dohán O,Nicola JP,Reyna-Neyra A,Follenzi A,Dadachova E,Eskandari S,Amzel LM,Carrasco N||Proceedings of the National Academy of Sciences of the United States of America (108:17933)||2011|
A role for mineralocorticoid receptors in the physiology of the ovine fetus: effects on ACTH and lung liquid composition.
MA3-929 was used in western blot to investigate the effect of mineralocorticoid receptors on plasma ACTH and lung fluid composition during lung development
|Keller-Wood M,Wood CE,McCartney J,Jesse NM,Perrone D||Pediatric research (69:491)||2011|
Influence of renal compensatory hypertrophy on mitochondrial energetics and redox status.
MA3-929 was used in western blot to investigate the molecular mechanism of renal compensatory hypertrophy in a uninephrectomized rat model
|Benipal B,Lash LH||Biochemical pharmacology (81:295)||2011|
Syntaxin 4 is required for acid sphingomyelinase activity and apoptotic function.
MA3-929 was used in western blot to study the activation of acid sphingomyelinase activity by syntaxin 4.
|Perrotta C,Bizzozero L,Cazzato D,Morlacchi S,Assi E,Simbari F,Zhang Y,Gulbins E,Bassi MT,Rosa P,Clementi E||The Journal of biological chemistry (285:40240)||2010|
The plasma membrane redox system is impaired by amyloid ß-peptide and in the hippocampus and cerebral cortex of 3xTgAD mice.
MA3-929 was used in western blot to investigate the effect of amyloid beta-peptide on plasma membrane redox system
|Hyun DH,Mughal MR,Yang H,Lee JH,Ko EJ,Hunt ND,de Cabo R,Mattson MP||Experimental neurology (225:423)||2010|
Sarcolemmal ATP-sensitive K(+) channels control energy expenditure determining body weight.
MA3-929 was used in western blot to investigate the role of Sarcolemmal ATP-sensitive K(+) channels in body weight determination and obesity
|Alekseev AE,Reyes S,Yamada S,Hodgson-Zingman DM,Sattiraju S,Zhu Z,Sierra A,Gerbin M,Coetzee WA,Goldhamer DJ,Terzic A,Zingman LV||Cell metabolism (11:58)||2010|
Regulation of Na,K-ATPase subunit abundance by translational repression.
MA3-929 was used in western blot to study how translational repression regulates alpha and beta subunit of Na, K-ATPase's expression
|Clifford RJ,Kaplan JH||The Journal of biological chemistry (284:22905)||2009|
Is the fetal lung a mineralocorticoid receptor target organ? Induction of cortisol-regulated genes in the ovine fetal lung, kidney and small intestine.
MA3-929 was used in western blot to study the function of mineralocorticoid receptor in fetal lungs
|Keller-Wood M,von Reitzenstein M,McCartney J||Neonatology (95:47)||2009|
beta-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells.
MA3-929 was used in western blot to study the effect of beta-subunit overexpression on Na-K-ATPase complex stoichemistry in Madin-Darby canine kidney cells.
|Clifford RJ,Kaplan JH||American journal of physiology. Renal physiology (295:F1314)||2008|
Selective basolateral localization of overexpressed Na-K-ATPase beta1- and beta2- subunits is disrupted by butryate treatment of MDCK cells.
MA3-929 was used in western blot to investigate the distribution of the sodium/potassium-ATPase beta 1- and beta 2-subunit isoforms stably expressed in MDCK cells and regulation by tetracycline
|Laughery MD,Clifford RJ,Chi Y,Kaplan JH||American journal of physiology. Renal physiology (292:F1718)||2007|
A Ca2+-binding domain in RyR1 that interacts with the calmodulin binding site and modulates channel activity.
MA3-929 was used in western blot to study the interaction of a fragment of RyR1 (R4064-C4210) with Cav1.1
|Xiong L,Zhang JZ,He R,Hamilton SL||Biophysical journal (90:173)||2006|
Coenzyme Q distribution in HL-60 human cells depends on the endomembrane system.
MA3-929 was used in western blot to study the role of endomembrane system in coenzyme Q distribution in HL-60 human cells
|Fernández-Ayala DJ,Brea-Calvo G,López-Lluch G,Navas P||Biochimica et biophysica acta (1713:129)||2005|
|Non-human primate||Not Cited||
Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin.
MA3-929 was used in western blot to locate iota b (Ib) oligomers in detergent-resistant membrane microdomains (DRMs) extracted from Vero cells
|Hale ML,Marvaud JC,Popoff MR,Stiles BG||Infection and immunity (72:2186)||2004|
Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome.
MA3-929 was used in western blot to investigate the ability of 7-dehydrocholesterol to replace cholesterol in membrane rafts.
|Keller RK,Arnold TP,Fliesler SJ||Journal of lipid research (45:347)||2004|
Mutational analysis of alpha-beta subunit interactions in the delivery of Na,K-ATPase heterodimers to the plasma membrane.
MA3-929 was used in western blot to study the transport of sodium/potassium ATPase heterodimers to plasma membrane.
|Laughery MD,Todd ML,Kaplan JH||The Journal of biological chemistry (278:34794)||2003|
Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors.
MA3-929 was used in western blot to investigate the isometric force and endurance in the muscles of mice deficient in TRalpha1 or TRbeta.
|Johansson C,Lunde PK,Gothe S,Lannergren J,Westerblad H||The Journal of physiology (547:789)||2003|
Functional properties of the copper-transporting ATPase ATP7B (the Wilson's disease protein) expressed in insect cells.
MA3-929 was used in western blot to detect the expression of Na,K-ATPase
|Tsivkovskii R,Eisses JF,Kaplan JH,Lutsenko S||The Journal of biological chemistry (277:976)||2002|
Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure.
MA3-929 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle
|Lunde PK,Dahlstedt AJ,Bruton JD,Lännergren J,Thorén P,Sejersted OM,Westerblad H||Circulation research (88:1299)||2001|
WFS1 (Wolfram syndrome 1) gene product: predominant subcellular localization to endoplasmic reticulum in cultured cells and neuronal expression in rat brain.
MA3-929 was used in western blot to investigate the role of the WFS1 protein in maintaining certain populations of neuronal and endocrine cells.
|Takeda K,Inoue H,Tanizawa Y,Matsuzaki Y,Oba J,Watanabe Y,Shinoda K,Oka Y||Human molecular genetics (10:477)||2001|
Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice.
MA3-929 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function
|Johansson C,Lännergren J,Lunde PK,Vennström B,Thorén P,Westerblad H||American journal of physiology. Regulatory, integrative and comparative physiology (278:R598)||2000|
Substitution of glutamic 779 with alanine in the Na,K-ATPase alpha subunit removes voltage dependence of ion transport.
MA3-929 was used in western blot to investigate the importance of Glu 779 in functional properties of sodium/potassium-ATPase alpha 1
|Argüello JM,Peluffo RD,Feng J,Lingrel JB,Berlin JR||The Journal of biological chemistry (271:24610)||1996|
Interactions of phosphorylation and dimerizing domains of the alpha-subunits of Na+/K(+)-ATPase.
MA3-929 was used in western blot to identify the dimerization domain of sodium/potassium-ATPase, which is regulated by the corresponding phosphorylation status
|Ganjeizadeh M,Zolotarjova N,Huang WH,Askari A||The Journal of biological chemistry (270:15707)||1995|
|Non-human primate||Not Cited||
EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells.
MA3-929 was used in immunocytochemistry to investigate the effect of EGF on claudin-2 and -4 expression in MDCK cells
|García-Hernández V,Flores-Maldonado C,Rincon-Heredia R,Verdejo-Torres O,Bonilla-Delgado J,Meneses-Morales I,Gariglio P,Contreras RG||Journal of cellular physiology (230:105)||2015|
Ouabain modulates ciliogenesis in epithelial cells.
MA3-929 was used in immunocytochemistry to investigate the effect of ouabain on cilia development in epithelial cells
|Larre I,Castillo A,Flores-Maldonado C,Contreras RG,Galvan I,Muñoz-Estrada J,Cereijido M||Proceedings of the National Academy of Sciences of the United States of America (108:20591)||2011|
Carboxypeptidase O is a glycosylphosphatidylinositol-anchored intestinal peptidase with acidic amino acid specificity.
MA3-929 was used in immunocytochemistry to investigate the structural and functional properties of carboxypeptidase O
|Lyons PJ,Fricker LD||The Journal of biological chemistry (286:39023)||2011|
Rotavirus NSP4: Cell type-dependent transport kinetics to the exofacial plasma membrane and release from intact infected cells.
MA3-929 was used in immunocytochemistry to study the intracellular transport of rotavirus NSP4 and its release from intact cells
|Gibbons TF,Storey SM,Williams CV,McIntosh A,Mitchel DM,Parr RD,Schroeder ME,Schroeder F,Ball JM||Virology journal (8:null)||2011|
Differential subcellular distribution of endosomal compartments and the dopamine transporter in dopaminergic neurons.
MA3-929 was used in immunocytochemistry to study the subcellular distribution and functions of endosomal compartments and dopamine transporter in dopaminergic neurons
|Rao A,Simmons D,Sorkin A||Molecular and cellular neurosciences (46:148)||2011|
Mineralocorticoid receptor expression in late-gestation ovine fetal lung.
MA3-929 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in the lung of late-gestation sheep fetus
|Keller-Wood M,Wood CE,Hua Y,Zhang D||Journal of the Society for Gynecologic Investigation (12:84)||2005|
Polyuria and impaired renal blood flow after asphyxia in preterm fetal sheep.
MA3-929 was used in immunohistochemistry to assess the presence of renal damage in preterm fetal sheep after asphyxia
|Quaedackers JS,Roelfsema V,Hunter CJ,Heineman E,Gunn AJ,Bennet L||American journal of physiology. Regulatory, integrative and comparative physiology (286:R576)||2004|
Action of palytoxin on apical H+/K+-ATPase in rat colon.
MA3-929 was used in immunohistochemistry to study how palytoxin affects the hydrogen/potassium-ATPase from the rat colon
|Scheiner-Bobis G,Hübschle T,Diener M||European journal of biochemistry (269:3905)||2002|
Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?
MA3-929 was used in blocking or activating experiment to study the relationship of the cardiac B-type Ca(2+) channel to the plasma membrane Ca(2+)-ATPase pump
|Antoine S,Pinet C,Coulombe A||The Journal of membrane biology (179:37)||2001|