Immunofluorescent analysis of Sodium/Potassium ATPase alpha using Sodium/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product# MA3-928 ) shows staining in MCF-7 Cells. Sodium/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodium/Potassium ATPase alpha (Product# MA3-928 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Dog, Chicken, Human, Mouse, Sheep, Pig, Rat|
|Published species reactivity||Dog, Rat, Pig, Sheep, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified alpha sodium/potassium ATPase from sheep kidney.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-928 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.
MA3-928 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha sodium/potassium ATPase from MDCK cell extract. Immunohistochemical staining of alpha sodium/potassium ATPase in porcine heart with MA3-928 yields a pattern consistent with the plasma membrane localization.
The MA3-928 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The subcellular localization of GABA transporters and its implication for seizure management.
MA3-928 was used in western blot to study seizure management by subcellular localization of GABA transporters
|Madsen KK,Hansen GH,Danielsen EM,Schousboe A||Neurochemical research (40:410)||2015|
Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins.
MA3-928 was used in western blot to evaluate a new gene transfection strategy based on a modified retrovirus glycoprotein
|Li F,Ryu BY,Krueger RL,Heldt SA,Albritton LM||Journal of virology (86:373)||2012|
POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.
MA3-928 was used in western blot to investigate the function of POST in STIM1 localization
|Krapivinsky G,Krapivinsky L,Stotz SC,Manasian Y,Clapham DE||Proceedings of the National Academy of Sciences of the United States of America (108:19234)||2011|
Presenilins and gamma-secretase inhibitors affect intracellular trafficking and cell surface localization of the gamma-secretase complex components.
MA3-928 was used in western blot to study the role of presenilins in intracellular trafficking of the gamma-secretase components.
|Wang H,Luo WJ,Zhang YW,Li YM,Thinakaran G,Greengard P,Xu H||The Journal of biological chemistry (279:40560)||2004|
Identification of a critical basic residue on the ecotropic murine leukemia virus receptor.
MA3-928 was used in western blot to identify the critical residues in ecotropic murine leukemia virus receptor influencing MLV binding and infection.
|Qian Z,Donald R,Wang H,Chen Q,Albritton LM||Journal of virology (77:8596)||2003|
Coronin is involved in uptake of Mycobacterium bovis BCG in human macrophages but not in phagosome maintenance.
MA3-928 was used in western blot to study the role of coronin in Mycobacterium bovis BCG infection
|Schüller S,Neefjes J,Ottenhoff T,Thole J,Young D||Cellular microbiology (3:785)||2001|
Plasma membrane depolarization without repolarization is an early molecular event in anti-Fas-induced apoptosis.
MA3-928 was used in western blot to investigate the potential movement of monovalent ions in Jurkat cells in the early stage of anti-Fas-induced apoptosis.
|Bortner CD,Gomez-Angelats M,Cidlowski JA||The Journal of biological chemistry (276:4304)||2001|
Loss of cyclosporin and azidopine binding are associated with altered ATPase activity by a mutant P-glycoprotein with deleted phe(335).
MA3-928 was used in western blot to investigate the phenotype caused by a P-glycoprotein with a deletion mutation of Phe335 and the possible roles of Phe335 in forming the major binding sites for cyclosporins and azidopine
|Chen GK,Lacayo NJ,Durán GE,Cohen D,Sikic BI||Molecular pharmacology (57:769)||2000|
Glut-1 and hexokinase expression: relationship with 2-fluoro-2-deoxy-D-glucose uptake in A431 and T47D cells in culture.
MA3-928 was used in western blot to study the glut-1 and hexokinase expression in six different cancer cell lines
|Aloj L,Caracó C,Jagoda E,Eckelman WC,Neumann RD||Cancer research (59:4709)||1999|
Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library.
MA3-928 was used in western blot to investigate the amino acid sequence of the surface-exposed epitope in sodium/potassium-ATPase's nucleotide-binding domain
|Malik B,Jamieson GA,Ball WJ||Protein science : a publication of the Protein Society (2:2103)||1993|
Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases.
MA3-928 was used in immunocytochemistry to study of Flotillin-2 expression from a cell model to human tissue in health and inflammatory bowel disease
|Gauss A,Buchholz I,Zahn A,Schmitz G,Stremmel W,Fuellekrug J,Ehehalt R||International journal of medical sciences (10:1259)||2013|
Ion transport and barrier function in a telomerase-immortalized human nondysplastic, Barrett's cell line (BAR-T).
MA3-928 was used in immunocytochemistry to evaluate an immortalized human nondysplastic Barrett cell line for the study of epithelial functions
|Jovov B,Orlando GS,Tobey NA,Brown KL,Djukic Z,Carson JL,Brighton LE,Orlando RC||Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus (22:386)||2009|
Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G.
MA3-928 was used in immunocytochemistry to investigate the role of 190-kDa ankyrin-G in the assembly of lateral membranes of bronchial epithelial cells.
|Kizhatil K,Bennett V||The Journal of biological chemistry (279:16706)||2004|
Intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.
MA3-928 was used in immunocytochemistry to study the intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.
|Low SH,Miura M,Roche PA,Valdez AC,Mostov KE,Weimbs T||Molecular biology of the cell (11:3045)||2000|
Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet.
MA3-928 was used in flow cytometry to study the role of the rumenal vacuolar H(+)-ATPase in the response of ovine rumen epithelial cells to an energy-rich dietary regimen
|Kuzinski J,Zitnan R,Albrecht E,Viergutz T,Schweigel-Röntgen M||American journal of physiology. Regulatory, integrative and comparative physiology (303:R909)||2012|
Early uptake and continuous accumulation of thallium-201 chloride in a benign mixed tumor of soft tissue: case report.
MA3-928 was used in immunohistochemistry to report a clinical case of benign mixed tumor of soft tissu with unique Tl-201 scintigraphic features
|Nagata S,Jin YF,Yoshizato K,Kitamura M,Iizuka N,Song M,Tomoeda M,Yuki M,Kubo C,Yoshizawa H,Outani H,Hamada K,Araki N,Funauchi M,Tomita Y||Diagnostic pathology (5:null)||2010|
Contraceptive steroids from pharmaceutical waste perturbate junctional communication in Sertoli cells.
MA3-928 was used in immunohistochemistry to study the effect of contraceptive steroids on gap junction activity in Sertoli cells
|Tramoni M,Gilleron J,Tahiri K,Carette D,Corvol MT,Segretain D,Pointis G,Savouret JF||Biochimie (91:1366)||2009|
Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.
MA3-928 was used in immunohistochemistry to study the localization of the leptin and the obesity receptor in the small intestine and colon.
|Hansen GH,Niels-Christiansen LL,Danielsen EM||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (56:677)||2008|