|Tested species reactivity||Bovine, Dog, Hamster, Human, Pig, Rabbit, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Clone||464.8 (aka 8A)|
|Immunogen||Purified Na,K-ATPase from rabbit renal outer medulla.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody does not react with mouse samples.
Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and Beta subunit. The Ã¡-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide is glycosylated and appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase Beta-1 subunit is expressed, exclusively, in epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Na¿, K¿-ATPase and Ca²¿-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta.
MA1-16732 was used in immunohistochemistry - paraffin section and western blot to evaluate the effect of preeclampsia on the activity and expression of the ion pump ATPases in syncytiotrophoblast
|Abad C,Vallejos C,De Gregorio N,Díaz P,Chiarello DI,Mendoza M,Piñero S,Proverbio T,Botana D,Rojas P,Riquelme G,Proverbio F,Marín R||Hypertension in pregnancy (34:65)||2015|