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Invitrogen
Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)
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FIGURE: 1 / 34
Sodium/Calcium Exchanger Antibody (MA3-926) in ICC/IF
Immunofluorescence analysis of Sodium/Calcium Exchanger was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Sodium/Calcium Exchanger Monoclonal Antibody (C2C12) (Product # MA3-926) at 1:200 dilution ... View More
Product Details
MA3-926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat
Published species
Bovine,
Dog,
Guinea pig,
Human,
Marsupial,
Mouse,
Pig,
Rabbit,
Rat,
Rodent
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
C2C12
Immunogen
Purified canine cardiac sodium/calcium exchanger.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.
MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.
The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.
Target Information
Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cardiac sarcolemmal sodium-calcium exchanger; isoform NCX1.13; isoform NCX1.3; isoform NCX1.9; Na(+)/Ca(2+)-exchange protein 1; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; Na-Ca exchanger; Na/Ca exchanger; NKX; sodium-calcium exchanger; sodium/calcium exchanger; Sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; solute carrier family 8 (sodium/calcium exchanger), member 1; Solute carrier family 8 member 1; solute carrier family 8, member 1
Gene Aliases: AI852629; AV344025; CNC; D930008O12Rik; NACA; Ncx; NCX1; SLC8A1
UniProt ID: (Dog) P23685, (Human) P32418, (Mouse) P70414, (Rat) Q01728, (Guinea pig) P48766
Entrez Gene ID: (Dog) 475738, (Rabbit) 100328570, (Human) 6546, (Mouse) 20541, (Rat) 29715, (Guinea pig) 100135620
calcium:sodium antiporter activity
calcium ion binding
calmodulin binding
ankyrin binding
calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration
protein binding
cytoskeletal protein binding
ion channel binding
antiporter activity
metal ion binding
calcium:sodium antiporter activity involved in regulation of cardiac muscle cell membrane potential
secondary carrier transporter
cellular sodium ion homeostasis
cell communication
positive regulation of bone mineralization
sodium ion transmembrane transport
response to muscle stretch
calcium ion import
calcium ion transmembrane transport
sodium ion import across plasma membrane
positive regulation of the force of heart contraction
regulation of postsynaptic cytosolic calcium ion concentration
regulation of the force of heart contraction
regulation of heart rate
regulation of sodium ion transport
ion transport
muscle contraction
response to nutrient
response to glucose
regulation of cell communication by electrical coupling
positive regulation of fibroblast migration
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of cardiac muscle contraction by calcium ion signaling
vascular smooth muscle contraction
telencephalon development
response to ATP
cellular response to reactive oxygen species
response to immobilization stress
response to drug
response to hydrogen peroxide
relaxation of smooth muscle
negative regulation of cytosolic calcium ion concentration
cardiac muscle cell development
calcium ion homeostasis
relaxation of cardiac muscle
cardiac muscle contraction
cytosolic calcium ion transport
calcium ion transport into cytosol
cellular response to caffeine
cellular response to cAMP
sodium ion export
cellular response to hypoxia
membrane depolarization during cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
sodium ion import
calcium ion export
regulation of cardiac conduction
response to hypoxia
embryonic placenta development
heart morphogenesis
transport
sodium ion transport
calcium ion transport
cellular calcium ion homeostasis
positive regulation of cytosolic calcium ion concentration
post-embryonic development
embryonic heart tube development
muscle fiber development
regulation of calcium ion transport
transmembrane transport
regulation of cardiac muscle cell membrane potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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