Invitrogen

Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody (3L6F0)

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Sodium Potassium ATPase Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Sodium Potassium ATPase in C6 cells using a Sodium Potassium ATPase Recombinant Monoclonal antibody (Product # MA5-42645). Blue: DAPI for nuclear staining.

Sodium Potassium ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Sodium Potassium ATPase in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42645) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol..

Sodium Potassium ATPase Antibody in Western Blot (WB) — Western Blot analysis of Sodium Potassium ATPase in various cell lines using a Sodium Potassium ATPase Recombinant Monoclonal antibody (Product # MA5-42645) at a dilution of 1:100,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Product Details

MA5-42645

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50 - 1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	3L6F0
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (P05023). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.715 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive test controls include: HeLa, 293T, Mouse Liver. The target is usually found in the following locations: Cell membrane, Melanosome, Multi-pass membrane protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+/K+ transporting, alpha 4 polypeptide; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; ATPase, Na+K+ transporting, alpha 1; Na(+)/K(+) ATPase alpha-1 subunit; Na(+)/K(+) ATPase alpha-4 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase alpha 4 subunit; Na+/K+ ATPase, alpha-D polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na,K-ATPase subunit alpha-C; sodium pump 4; Sodium pump subunit alpha-1; Sodium pump subunit alpha-4; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-4; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-4 chain; Sodium/potassium-transporting ATPase subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-4

Gene Aliases: ATP1A1; ATP1A4; ATP1AL2; Atpa-1; BC010319; Nkaa1b

UniProt ID: (Human) P05023, (Human) Q13733, (Rat) Q64609, (Mouse) Q8VDN2, (Mouse) Q9WV27, (Rat) Q64541

Entrez Gene ID: (Human) 476, (Human) 480, (Rat) 24211, (Mouse) 11928, (Mouse) 27222, (Rat) 29132

Molecular Function: primary active transporter