|Tested species reactivity||Bovine, Dog, Fruit fly, Guinea pig, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast|
|Published species reactivity||Rat, Amphibian, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified Na,K-ATPase from rabbit renal outer medulla.|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Do not boil samples prior to Western Blot.
Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, wherease the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase alpha-1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A role for Na+,K+-ATPase ¿1 in regulating Rab27a localisation on melanosomes.
MA1-16731 was used in immunocytochemistry to analyze regulation of Rab27a localisation on melanosomes by Na+,K+-ATPase alpha1
|Booth AE,Tarafder AK,Hume AN,Recchi C,Seabra MC||PloS one (9:null)||2014|
Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.
MA1-16731 was used in immunohistochemistry and western blot to study the rat enamel cell expression of Na(+)/K(+)-ATPase subunits
|Wen X,Lacruz RS,Smith CE,Paine ML||European journal of oral sciences (122:21)||2014|
Altered Na+ transport after an intracellular alpha-subunit deletion reveals strict external sequential release of Na+ from the Na/K pump.
MA1-16731 was used in western blot to investigate the important role of the alpha-subunit in external release of sodium from the sodium/potassium pump
|Yaragatupalli S,Olivera JF,Gatto C,Artigas P||Proceedings of the National Academy of Sciences of the United States of America (106:15507)||2009|