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Immunofluorescent analysis of Sodium/Potassium ATPase beta using Anti-Sodium/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product# MA3-930) shows staining in MCF-7 Cells. Sodium/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase beta (Product# MA3-930) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Dog, Human, Mouse, Sheep, Pig|
|Published species reactivity||Dog, Fruit fly, Sheep, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified lamb kidney beta sodium/potassium ATPase.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
MA3-930 has been successfully used in Western blot and immunohistochemistry and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract. Immunohistochemical staining of the sodium/potassium ATPase with MA3-930 yields a pattern consistent with plasma membrane localization.
The MA3-930 antigen is purified lamb kidney sodium/potassium ATPase beta. This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific and developmental pattern of expression. The alpha 1 and beta mRNAs are present in all cell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The effects of knee injury on skeletal muscle function, Na+, K+-ATPase content, and isoform abundance.
MA3-930 was used in western blot to investigate the relationship between skeletal muscle Na(+), K(+)-ATPase (NKA) and knee injury
|Perry BD,Levinger P,Morris HG,Petersen AC,Garnham AP,Levinger I,McKenna MJ||Physiological reports (3:null)||2015|
Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.
MA3-930 was used in western blot to study the effects of speed endurance training and heavy resistance training on running performance improvement
|Skovgaard C,Christensen PM,Larsen S,Andersen TR,Thomassen M,Bangsbo J||Journal of applied physiology (Bethesda, Md. : 1985) (117:1097)||2014|
The effects of osteoarthritis and age on skeletal muscle strength, Na+-K+-ATPase content, gene and isoform expression.
MA3-930 was used in western blot to study how skeletal muscle strength and the expression of Na(+)/K(+)-ATPase isoforms are affected by age and osteoarthritis
|Perry BD,Levinger P,Serpiello FR,Caldow MK,Cameron-Smith D,Bartlett JR,Feller JA,Bergman NR,Levinger I,McKenna MJ||Journal of applied physiology (Bethesda, Md. : 1985) (115:1443)||2013|
Effect of intensified training on muscle ion kinetics, fatigue development, and repeated short-term performance in endurance-trained cyclists.
MA3-930 was used in western blot to study muscle ion kinetics, fatigue and repeat intense exercise performance in trained cyclists subjected to increased intesity/reduced volume training
|Gunnarsson TP,Christensen PM,Thomassen M,Nielsen LR,Bangsbo J||American journal of physiology. Regulatory, integrative and comparative physiology (305:R811)||2013|
Unchanged [3H]ouabain binding site content but reduced Na+-K+ pump α2-protein abundance in skeletal muscle in older adults.
MA3-930 was used in western blot to study the reduced abundance of the Na(+),K(+) pump alpha2 subunit in aged human skeletal muscle
|McKenna MJ,Perry BD,Serpiello FR,Caldow MK,Levinger P,Cameron-Smith D,Levinger I||Journal of applied physiology (Bethesda, Md. : 1985) (113:1505)||2012|
The 10-20-30 training concept improves performance and health profile in moderately trained runners.
MA3-930 was used in western blot to study the beneficial effects of intensive short-burst interval training on athletic performance and health parameters in moderately trained runners
|Gunnarsson TP,Bangsbo J||Journal of applied physiology (Bethesda, Md. : 1985) (113:16)||2012|
Four weeks of normobaric "live high-train low" do not alter muscular or systemic capacity for maintaining pH and K⁺ homeostasis during intense exercise.
MA3-930 was used in western blot to study the effects on systemic and muscular pH control capacity and K(+) homeostasis of four weeks of normobaric hypoxia
|Nordsborg NB,Siebenmann C,Jacobs RA,Rasmussen P,Diaz V,Robach P,Lundby C||Journal of applied physiology (Bethesda, Md. : 1985) (112:2027)||2012|
Impaired exercise performance and muscle Na(+),K(+)-pump activity in renal transplantation and haemodialysis patients.
MA3-930 was used in western blot to investigate the relationship between abnormal skeletal muscle Na+,K+-pumps and impaired exercise performance in hemodialysis patients and renal transplant recipients
|Petersen AC,Leikis MJ,McMahon LP,Kent AB,Murphy KT,Gong X,McKenna MJ||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (27:2036)||2012|
Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans.
MA3-930 was used in western blot to investigate the expression levels of Na+/K+-ATPase and phospholemman in patients with spinal cord injury
|Boon H,Kostovski E,Pirkmajer S,Song M,Lubarski I,Iversen PO,Hjeltnes N,Widegren U,Chibalin AV||American journal of physiology. Endocrinology and metabolism (302:E864)||2012|
Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.
MA3-930 was used in western blot to investigate the effect of exercise on the phosphorylation of skeletal phospholemman
|Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV||American journal of physiology. Endocrinology and metabolism (301:E456)||2011|
Relationship between performance at different exercise intensities and skeletal muscle characteristics.
MA3-930 was used in western blot to study the association of various skeletal muscle characteristics with exercise performance over a range of power outputs
|Iaia FM,Perez-Gomez J,Thomassen M,Nordsborg NB,Hellsten Y,Bangsbo J||Journal of applied physiology (Bethesda, Md. : 1985) (110:1555)||2011|
Effect of 2-wk intensified training and inactivity on muscle Na+-K+ pump expression, phospholemman (FXYD1) phosphorylation, and performance in soccer players.
MA3-930 was used in western blot to investigate the influence of intensified training and inactivity on specific gene expression in football players
|Thomassen M,Christensen PM,Gunnarsson TP,Nybo L,Bangsbo J||Journal of applied physiology (Bethesda, Md. : 1985) (108:898)||2010|
Ubiquitination participates in the lysosomal degradation of Na,K-ATPase in steady-state conditions.
MA3-930 was used in western blot to study the role of ubiquitination in lysosomal degradation of Na,K-ATPase in steady-state conditions
|Lecuona E,Sun H,Vohwinkel C,Ciechanover A,Sznajder JI||American journal of respiratory cell and molecular biology (41:671)||2009|
Regulation of Na,K-ATPase subunit abundance by translational repression.
MA3-930 was used in western blot to study how translational repression regulates alpha and beta subunit of Na, K-ATPase's expression
|Clifford RJ,Kaplan JH||The Journal of biological chemistry (284:22905)||2009|
beta-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells.
MA3-930 was used in western blot to study the effect of beta-subunit overexpression on Na-K-ATPase complex stoichemistry in Madin-Darby canine kidney cells.
|Clifford RJ,Kaplan JH||American journal of physiology. Renal physiology (295:F1314)||2008|
Reduced volume but increased training intensity elevates muscle Na+-K+ pump alpha1-subunit and NHE1 expression as well as short-term work capacity in humans.
MA3-930 was used in western blot to study the expression of sodium/potassium pump alpha1-subunit and NHE1 in endurance-trained runners
|Iaia FM,Thomassen M,Kolding H,Gunnarsson T,Wendell J,Rostgaard T,Nordsborg N,Krustrup P,Nybo L,Hellsten Y,Bangsbo J||American journal of physiology. Regulatory, integrative and comparative physiology (294:R966)||2008|
Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes.
MA3-930 was used in western blot to demonstrate that the upregulation of sodium/potassium-ATPase alpha mRNA play a role in responding to intense interval exercise and training in athletes
|Aughey RJ,Murphy KT,Clark SA,Garnham AP,Snow RJ,Cameron-Smith D,Hawley JA,McKenna MJ||Journal of applied physiology (Bethesda, Md. : 1985) (103:39)||2007|
Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle.
MA3-930 was used in western blot to study the role of prolonged submaximal exercise on the expression level of sodium/potassium-ATPase mRNA and protein in human skeletal muscle
|Murphy KT,Petersen AC,Goodman C,Gong X,Leppik JA,Garnham AP,Cameron-Smith D,Snow RJ,McKenna MJ||American journal of physiology. Regulatory, integrative and comparative physiology (290:R414)||2006|
|Fruit fly||Not Cited||
Oligomerization of the Na,K-ATPase in cell membranes.
MA3-930 was used in western blot to investigate the heterodimerization of the sodium/potassium ATPase in native cell membranes.
|Laughery M,Todd M,Kaplan JH||The Journal of biological chemistry (279:36339)||2004|
Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle.
MA3-930 was used in western blot to investigate the effect of acute exercise effects on sodium/potassium-ATPase isoform expression in human skeletal muscle.
|Murphy KT,Snow RJ,Petersen AC,Murphy RM,Mollica J,Lee JS,Garnham AP,Aughey RJ,Leppik JA,Medved I,Cameron-Smith D,McKenna MJ||The Journal of physiology (556:507)||2004|
Mutational analysis of alpha-beta subunit interactions in the delivery of Na,K-ATPase heterodimers to the plasma membrane.
MA3-930 was used in western blot to study the transport of sodium/potassium ATPase heterodimers to plasma membrane.
|Laughery MD,Todd ML,Kaplan JH||The Journal of biological chemistry (278:34794)||2003|
Na pump isoforms in human erythroid progenitor cells and mature erythrocytes.
Ma3-930 was used in western blot to profile the sodium pump isoforms in human erythroid progenitor cells and mature erythrocytes.
|Hoffman JF,Wickrema A,Potapova O,Milanick M,Yingst DR||Proceedings of the National Academy of Sciences of the United States of America (99:14572)||2002|
Plasma membrane depolarization without repolarization is an early molecular event in anti-Fas-induced apoptosis.
MA3-930 was used in western blot to investigate the potential movement of monovalent ions in Jurkat cells in the early stage of anti-Fas-induced apoptosis.
|Bortner CD,Gomez-Angelats M,Cidlowski JA||The Journal of biological chemistry (276:4304)||2001|
Subunit isoform selectivity in assembly of Na,K-ATPase α-β heterodimers.
MA3-930 was used in immunocytochemistry to study subunit isoform selectivity in the assembly of Na,K-ATPase alpha-beta heterodimers
|Tokhtaeva E,Clifford RJ,Kaplan JH,Sachs G,Vagin O||The Journal of biological chemistry (287:26115)||2012|
Molecular and functional studies of electrogenic Na(+) transport in the distal colon and rectum of young and elderly subjects.
MA3-930 was used in immunocytochemistry to study the age-related expression of apical sodium channels and basolateral sodium, potassium-ATPase in human distal colon and rectum.
|Greig ER,Mathialahan T,Boot-Handford RP,Sandle GI||Gut (52:1607)||2003|
adenosinetriphosphatase; ATPase beta subunit; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; Na+, K+-ATPase beta-subunit; Na+/K+ ATPase Beta; Na, K-ATPase beta-1 polypeptide; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; sodium/potassium-transporting ATPase subunit beta-1
ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1