|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(380) R N G T C T S R I T T L(391) of human SSTR1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA3-108 detects the Somatostatin Receptor 1 in human samples.
PA3-108 has been used successfully in Western blot, immunocytochemistry, and immunohistochemistry procedures. In Western blot analysis of SSTR1 transfected HEK-293 cells this antibody detects a ~52-63 kDa protein representing SSTR1.
The PA3-108 immunogen is a synthetic peptide corresponding to residues C(380) R N G T C T S R I T T L(391) of human SSTR1.
The Somatostatin Receptor Type 1 (SSTR1) has been reported at highest levels in the jejunum and stomach. In addition, SSTR1 has been shown to be expressed in adrenal, brain, eye, and panreas. ESTs have been isolated from normal placenta and colon cancer libraries.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Somatostatin receptor subtypes in human pheochromocytoma: subcellular expression pattern and functional relevance for octreotide scintigraphy.
PA3-108 was used in immunohistochemistry to investigate the cellular distribution of five somatostatin receptor subtypes in human pheochromocytoma
|Mundschenk J,Unger N,Schulz S,Höllt V,Schulz S,Steinke R,Lehnert H||The Journal of clinical endocrinology and metabolism (88:5150)||2003|