|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C Q(385) E R P P S R V A(393) of human SSTR3.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
PA3-110 detects the Somatostatin Receptor 3 in human samples.
PA3-110 has been used successfully in Western blot, immunocytochemistry, and immunohistochemistry procedures. In Western blot analysis of SSTR3 transfected HEK-293 cells this antibody detects a ~60-70 kDa protein representing SSTR3.
The PA3-110 immunogen is a synthetic peptide corresponding to residues C Q(385) E R P P S R V A(393) of human SSTR3.
Expression of Somatostatin Receptor Type 3 (SSTR3) has been reported in brain, breast, eye, gastrointestinal tract, pancreas, and thyroid.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Somatostatin receptor subtypes in human pheochromocytoma: subcellular expression pattern and functional relevance for octreotide scintigraphy.
PA3-110 was used in immunohistochemistry and western blot to investigate the cellular distribution of five somatostatin receptor subtypes in human pheochromocytoma
|Mundschenk J,Unger N,Schulz S,Höllt V,Schulz S,Steinke R,Lehnert H||The Journal of clinical endocrinology and metabolism (88:5150)||2003|
|Not Applicable||Not Cited||
Identification of somatostatin receptor subtypes 1, 2A, 3, and 5 in neuroendocrine tumours with subtype specific antibodies.
PA3-110 was used in immunohistochemistry and western blot to investigate the distribution of sstr1, 2A, 3, and 5 in neuroendocrine tumours
|Kulaksiz H,Eissele R,Rössler D,Schulz S,Höllt V,Cetin Y,Arnold R||Gut (50:52)||2002|