Invitrogen

Sp56 Monoclonal Antibody (7C5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

4 References

View all (7) Sp56 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (4)
Advanced Verification (1)

Sp56 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Sp56 Antibody (MA1-10866) in ICC/IF

Biology of reproduction 2015 - Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunocytochemistry

Sp56 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Sp56 Antibody — Fig 7 Chemical inhibition of GRASP55 affects spermatid differentiation in vivo. (A-B) Flow cytometry profiles of DAPI-stained germ cells isolated from testes of Gorasp2+/+ and Gorasp2-/- 35-days old mice (A) and 27-days old mice treated with vehicle or Graspin for two weeks (B). ES: elongated spermatids, RS: round spermatids, 2C: spermatogonia, 4C: primary spermatocytes. (C) Quantification of germ cell numbers of 27-days old mice treated with Graspin for two weeks and analyzed two days after the last Graspin injection. Vehicle, n = 10; Graspin, n = 10. Student’s unpaired t-test; *: P<0.05. (D) Confocal images of JAM-C, GRASP55 and DAPI staining of seminiferous tubule sections of 43-days old mice treated for 16 days with vehicle or Graspin. Differentiation stages of seminiferous tubules are indicated. High magnification pictures on the left panel highlight the loss of co-polarized localization of GRASP55 and JAM-C in developing round spermatids. Scale bars: left panels: 50 µm; right panels: 10 µm. (E) Quantification of co-polarized JAM-C and GRASP55 staining occurrence in control and treated mice expressed as the percentage of round spermatids showing a close apposition between GRASP55 and JAM-C stainings. Results obtained upon quantification of co-polarized staining occurrences in seminiferous tubules at stage V-VI (n = 415 counted events, n = 4 mice, 2 tissue sections/mice) and VIII (n = 498 counted events, n = 6 mice, 2 tissue sections/mice) are shown. Student’s unpaired t-test; **: P<0.01, ***: P<0.001. (F) Confocal images of SP56 and DAPI stainin {TM}

Product Details

MA1-10866

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

1-10 µg/mL

Product Specifications

Species Reactivity

Hamster, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7C5

Immunogen

Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1087440

Product Specific Information

MA1-10866 detects mouse sperm protein sp56 from mouse and hamster samples. MA1-10866 does not cross-react with human sperm.

MA1-10866 has been successfully used in ELISA, Western blot, and immunohistochemistry applications. MA1-18066 will react with denatured, non-reduced sp56 but not with denatured, reduced sp56.

The MA1-10866 immunogen is a mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transient reduction of DNA methylation at the onset of meiosis in male mice.

Epigenetics & chromatin

Gaysinskaya V,Miller BF,De Luca C,van der Heijden GW,Hansen KD,Bortvin A

Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunofluorescence

Wed Apr 04 00:00:00 EDT 2018

Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis.

PLoS genetics

Cartier-Michaud A,Bailly AL,Betzi S,Shi X,Lissitzky JC,Zarubica A,Sergé A,Roche P,Lugari A,Hamon V,Bardin F,Derviaux C,Lembo F,Audebert S,Marchetto S,Durand B,Borg JP,Shi N,Morelli X,Aurrand-Lions M

Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunofluorescence

Thu Jun 01 00:00:00 EDT 2017

Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility.

Biology of reproduction

Bhattacharjee R,Goswami S,Dudiki T,Popkie AP,Phiel CJ,Kline D,Vijayaraghavan S

Published figure using Sp56 monoclonal antibody (Product # MA1-10866) in Immunofluorescence

Sun Mar 01 00:00:00 EST 2015

USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET.

Reproduction (Cambridge, England)

Berruti G,Paiardi C

MA1-10866 was used in immunocytochemistry to study the biogenesis of the acrosome

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: AC122773.1; sperm fertilization protein 56; zona pellucida sperm-binding protein 3 receptor

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Gene Aliases: AV264399; SP56

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Entrez Gene ID: (Mouse) 22789

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Function(s)

protein binding

Process(es)

single fertilization binding of sperm to zona pellucida

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Sp56 Monoclonal Antibody (7C5)

Sp56 Antibody (MA1-10866) in ICC/IF

Product Details

MA1-10866

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Bioinformatics

Performance Guarantee

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