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NeoBiotechnologies
Spectrin beta III (SPTBN2) Monoclonal Antibody (SPTBN2, 1584)
FIGURE: 1 / 9
Spectrin beta III (SPTBN2) Antibody (6712-MSM4-P1ABX) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of MeOH-fixed HeLa cells using Spectrin beta III (SPTBN2) Monoclonal Antibody (Product # 6712-MSM4-P1ABX) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)..
Product Details
6712-MSM4-P1ABX
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SPTBN2, 1584
Immunogen
Recombinant fragment (around aa356-475) of human SPTBN2 protein
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta SpIII sigma 1; Beta-III spectrin; beta-spectrin 3; glutamate transporter EAAT4-associated protein 41; Spectrin Beta; spectrin beta 3; spectrin beta chain, brain 2; Spectrin beta chain, non-erythrocytic 2; spectrin beta III sigma 2; spectrin, beta, non-erythrocytic 2; spectrin, non-erythroid beta chain 2; spectrin-like protein GTRAP41; Spinocerebellar ataxia 5 protein; SPNB-3
Gene Aliases: GTRAP41; KIAA0302; mKIAA0302; SCA5; SCAR14; Spnb3; SPTBN2
UniProt ID: (Human) O15020
Entrez Gene ID: (Human) 6712, (Mouse) 20743, (Rat) 29211
synapse assembly
synaptic vesicle exocytosis
vesicle-mediated transport
cerebellar Purkinje cell layer morphogenesis
adult behavior
multicellular organism growth
MAPK cascade
ER to Golgi vesicle-mediated transport
cytoskeleton organization
axon guidance
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of GTPase activity
actin filament capping
cellular response to ethanol
cell-cell adhesion
synaptic vesicle docking
regulation of calcium ion-dependent exocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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