|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain (Human).|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with ammonium sulfate|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-91103 detects Spectrin non erythroid from human samples.
MA1-91103 has been successfully used in immunofluorescence, immunohistochemistry (frozen tissue), ELISA, and Western blot applications.
The MA1-91103 immunogen is recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain (Human).
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of ¿-II-spectrin breakdown products in the prediction of the severity and clinical outcome of acute traumatic brain injury.
MA1-91103 was used in western blot to characterize the prediction of the severity and clinical outcome of acute traumatic brain injury and the role of alpha-II-spectrin breakdown products
|Chen S,Shi Q,Zheng S,Luo L,Yuan S,Wang X,Cheng Z,Zhang W||Experimental and therapeutic medicine (11:2049)||2016|