Immunohistochemical analysis of Sphingolipid Receptor Edg3/S1P3 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top left) and a Sphingolipid Receptor Edg3/S1P3 polyclonal antibody (Product # PA5-23225) (bottom left, right) at 5 ug/ml.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (326 SPIQPALDPSRSKSSSSNNSS 346) of Sphingolipid Receptor Edg3/S1P3 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1.0-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Edg3 is a member of the EDG family of receptors which acts as a major functional receptor for a potent, extracellular lysolipid phosphoric acid mediator, S1P. It is the major S1P receptor subtype in the cardiovascular system, where it mediate the effects of S1P released from platelets, and possibly other tissues (such as brain). The receptor thus enhances endothelial and vascular smooth muscle cell proliferation and migration, playing a key role in developmental and pathological angiogenesis. The protein also mediates S1P-induced inflammatory response genes expression and can be used as drug targets to treat severe inflammation. Edg3 is expressed in many cell types, including endothelial and vascular smooth muscle cells and most abundantly in heart, placenta, kidney, and liver. Human Edg3 gene is mapped to chromosome 9q22.1-q22.2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.