|Tested species reactivity||Dog, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (15 IVLHYNYTGKLRGARYQ 31) of Sphingolipid Receptor Edg8/S1P5 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Sphingosine 1-phosphate receptor Edg8 is a multi-pass membrane protein belonging to the G-protein coupled receptor 1 family and coupled to both the G (i/0) alpha and G (12) subclass of heteromeric G-proteins. Edg8 acts as a receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) as well as for lysolipid mediator lysophosphatidic acid (LPA) and gives diverse physiological effect on most types of cells and tissues. Edg8 may also play a regulatory role in the transformation of radial glial cells into astrocytes and affect proliferative activity of these cells. It is widely expressed in white matter tracts of brain, spleen, peripheral blood leukocytes, placenta, lung, aorta, and fetal spleen. Over expression of Edg8 leads to a lymphoproliferative disorder often associated with the autoimmune disease known as leukemic large granular lymphocyte (LGL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.