|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a region between residue 334-384 of human sphingosine kinase 1 using the numbering given in entry NP_001136073.1|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||5-15 µg/mg lysate|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1 activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.