|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:50-1:200|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal of Sphingosine Kinase 2.|
|Purification||Antigen affinity chromatography|
|Contains||0.08% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is active recombinant sphingosine kinase enzyme.
Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cellcell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C76851; Fallotein; MGC102297; PDGF-C; Platelet-derived growth factor C; platelet-derived growth factor, C polypeptide; rScdfg; SCDGF; secretory growth factor-like protein; Spinal cord-derived growth factor; VEGF-E
Gene Aliases: 1110064L01Rik; AI647969; FALLOTEIN; PDGF-C; PDGFC; SCDGF; UNQ174/PRO200
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.