Western blot of Spondin-2/Mindin in A549 lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a Spondin-2/Mindin polyclonal antibody (Product# PA5-37919) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-RTRYVRVQPANNGSP, from the internal region near the C-terminus of SPON2 (aa301-315)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat and dog based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.