|Tested species reactivity||Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of mouse and rat Sprouty-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells. Four mammalian Sprouty homologues (Sprouty-1-4) have been identified. One study showed that overexpressed Sprouty-1 and -2 inhibits fibroblast growth factor and vascular endothelial growth factor-induced proliferation and differentiation by repressing pathways leading to MAP kinase activation. Sprouty proteins are also endogenous inhibitors of the Ras/MAP kinase pathway that play an important role in the remodeling of branching tissues, such as in development of the lung, kidney tubules, vascular system, and breast ducts. A recent study shows that Sprouty-1 and -2 may have tumor suppressing activity in the breast, and found that both proteins are consistently down-regulated in breast carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of MAP2K2/MEK2: a novel mechanism for a RASopathy?
40-1800 was used in western blot to describe the phenotype of seven patients with de novo deletions of chromosome 19p13.3
|Nowaczyk MJ,Thompson BA,Zeesman S,Moog U,Sanchez-Lara PA,Magoulas PL,Falk RE,Hoover-Fong JE,Batista DA,Amudhavalli SM,White SM,Graham GE,Rauen KA||Clinical genetics (85:138)||2014|
|Mouse||1:1000||Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program.||Lagha M,Kormish JD,Rocancourt D,Manceau M,Epstein JA,Zaret KS,Relaix F,Buckingham ME||Genes and development (22:1828)||2008|
|Dual effects of Sprouty1 on TCR signaling depending on the differentiation state of the T cell.||Choi H,Cho SY,Schwartz RH,Choi K||Journal of immunology (Baltimore, Md. : 1950) (176:6034)||2006|
Targeted Sprouty1 overexpression in cardiac myocytes does not alter myocardial remodeling or function.
40-1800 was used in western blot to test if up-regulation of Spry1 expression in cardiac myocytes inhibits ERK1/2 activation and tissue remodeling
|Charles NJ,Huebert RC,Lee S,Adhikari N,Polster S,Rider JE,Braunlin E,Mariash A,Robledo M,Schuweiler D,Hall JL||Molecular and cellular biochemistry (342:57)||2010|