|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The entire Srb4p protein (amino acid residues 1-687)|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4933 detects Srb4p in budding yeast samples.
PA1-4933 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~100 kDa protein (Srb4p) in yeast samples.
PA1-4933 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire Srb4p protein (amino acid residues 1-687)
Mediator is a protein complex which performs a very important role both for the transcription at the basal level which does not rely on transcription control factor and the transcription activating reaction that relies on the transcription control factor. It has the characteristic of binding to the 7 amino acid repeated structure (CTD) that exists in the C terminal of the biggest subunit of RNA polymerase II. 30 kinds of subunits have already being identified as its composing substance, but it is said that multiple kinds of complex with varied subunit compositions exist in the cells. Srb4p is one of the mediator subunit and is composed of 687 amino acid residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.