Western blot analysis of Staufen using A) 293 (input) (B) control rabbit IgG-IP (C) Stau1-IP (D) Post-IP lysate from control rabbit IgG-IP and E) Post-IP lysate from Stau1-IP. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Staufen polyclonal antibody (Product # PA5-28479) at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 379 and 577 of Human Staufen|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28479 targets Staufen in IP and WB applications and shows reactivity with Human and mouse samples.
The PA5-28479 immunogen is recombinant fragment corresponding to a region within amino acids 379 and 577 of Human Staufen.
Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.