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Western blot analysis of Streptavidin using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of strep-tagged protein expressing 293T cells. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Streptavidin monoclonal antibody (Product # MA5-17283) at a dilution of 1:5000.
|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Streptomyces avidinii Streptavidin|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17283 targets Streptavidin in ICC, IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-17283 immunogen is streptomyces avidinii Streptavidin.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~10-14 mol/l.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.