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|Host / Isotype||Not Applicable|
|Storage buffer||TBS, pH 8, with 50% glycerol, 0.1mM MgCl2, 0.1mM ZnCl2|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1 ug/ml|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.