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|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||the entire Sua7p protein (amino acid residues 1-346)|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4935 detects Sua7p in budding yeast samples.
PA1-4935 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~41 kDa protein (Sua7p) in yeast samples.
PA1-4935 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire Sua7p protein (amino acid residues 1-346).
The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance. Sua7p is the TFIIB of budding yeast and is composed of 346 amino acid residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.