|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human protein purified from E.coli.|
|Storage buffer||HEPES with 0.01% BSA, 0.15M NaCl, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||1 µl|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa or 293T cells.
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential regulation of glutamate transporter subtypes by pro-inflammatory cytokine TNF-¿ in cortical astrocytes from a rat model of amyotrophic lateral sclerosis.
LF-PA0013 was used in western blot to assess the role of TNF alpha in the regulation of glutamate transporter subtypes.
|Dumont AO,Goursaud S,Desmet N,Hermans E||PloS one (9:null)||2014|
Pretreatment with interferon-gamma protects microglia from oxidative stress via up-regulation of Mn-SOD.
LF-PA0013 was used in western blot to investigate the protective effect of interferon gamma on microglia against oxidative stress
|Chen X,Choi IY,Chang TS,Noh YH,Shin CY,Wu CF,Ko KH,Kim WK||Free radical biology and medicine (46:1204)||2009|