|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant pro-surfactant protein B|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-13975 targets Surfactant Protein B (Precursor) in IF and IHC (P) applications and shows reactivity with Human and Rat samples.
The MA5-13975 immunogen is recombinant pro-surfactant protein B.
Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical staining of surfactant proteins A and B in skin of psoriatic patients before and after narrow-band UVB phototherapy.
MA5-13975 was used in immunohistochemistry to study the effect of narrow-band UVB phototherapy on the immunohistochemical expresson of surfactant proteins A and B in psoriasis patients
|Aiad HA,El-Farargy SM,Soliman MM,El-Wahed Gaber MA,El-Aziz Othman SA||American journal of clinical dermatology (13:341)||2012|
Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect.
MA5-13975 was used in immunohistochemistry to investigate the role of TTF1 in lung surfactant homeostasis and respiratory system integrity
|Peca D,Petrini S,Tzialla C,Boldrini R,Morini F,Stronati M,Carnielli VP,Cogo PE,Danhaive O||Respiratory research (12:null)||2011|
TTF-1 expression in ovarian and uterine epithelial neoplasia and its potential significance, an immunohistochemical assessment with multiple monoclonal antibodies and different secondary detection systems.
MA5-13975 was used in immunohistochemistry to examine the diagnostic significance of thyroid transcription factor-1 in ovarian and uterine epithelial neoplasia
|Zhang PJ,Gao HG,Pasha TL,Litzky L,Livolsi VA||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (28:10)||2009|
Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma.
MA5-13975 was used in immunohistochemistry to report a clinical case of basaloid adenocarcinoma
|Marci V,Volante M,Cappia S,Righi L,Novello C,Scagliotti GV,Brambilla E,Papotti M||Virchows Archiv : an international journal of pathology (451:729)||2007|