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Immunofluorescent analysis of Survivin (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Survivin monoclonal antibody (Product # MA5-11680) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rabbit, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full length recombinant human survivin protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11680 targets Survivin in ICC/IF and IHC (P) applications and shows reactivity with Human and Rat samples.
The MA5-11680 immunogen is full length recombinant human survivin protein.
Research Areas: Apoptosis; Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2/M phase. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Arsenic trioxide treatment of rabbit liver VX-2 carcinoma via hepatic arterial cannulation-induced apoptosis and decreased levels of survivin in the tumor tissue.
MA5-11680 was used in immunohistochemistry to study the role of survivin in rabbit liver VX-2 carcinoma apotosis induced by arsenic trioxide
|Li H,Gong J,Jiang X,Shao H||Croatian medical journal (54:12)||2013|
Prognostication of soft tissue sarcomas based on chromosome 17q gene and protein status: evaluation of TOP2A, HER-2/neu, and survivin.
MA5-11680 was used in immunohistochemistry to study the prognostic value of chromosome 17q gene and protein status in soft tissue carcinoma
|da Cunha IW,De Brot L,Carvalho KC,Rocha RM,Fregnani JH,Falzoni R,Ferreira Fde O,Aguiar S,Lopes A,Muto NH,Reis LF,Soares FA,Vassallo J||Annals of surgical oncology (19:1790)||2012|
Norcantharidin inhibits growth of human gallbladder carcinoma xenografted tumors in nude mice by inducing apoptosis and blocking the cell cycle in vivo.
MA5-11680 was used in immunohistochemistry to investigate the inhibitory effect of norcantharidin on the tumor growth of human gallbladder carcinoma xenografted in nude mice in vivo
|Fan YZ,Zhao ZM,Fu JY,Chen CQ,Sun W||Hepatobiliary and pancreatic diseases international : HBPD INT (9:414)||2010|
Survivin expression quantified by Image Pro-Plus compared with visual assessment.
MA5-11680 was used in immunohistochemistry to compare immunohistochemical survivin expression quantified by Image Pro-Plus and visual assessment
|Wang CJ,Zhou ZG,Holmqvist A,Zhang H,Li Y,Adell G,Sun XF||Applied immunohistochemistry and molecular morphology : AIMM (17:530)||2009|
Significance of an exon 2 G4C14-to-A4T14 polymorphism in the p73 gene on survival in rectal cancer patients with or without preoperative radiotherapy.
MA5-11680 was used in immunohistochemistry to study if an exon 2 polymorphism in the p73 gene is related to survival in rectal cancer patients
|Lööf J,Pfeifer D,Adell G,Sun XF||Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology (92:215)||2009|
Deoxycholic acid promotes the growth of colonic aberrant crypt foci.
MA5-11680 was used in immunohistochemistry to investigate the effect of deoxycholic acid on the growth of aberrant crypt foci
|Flynn C,Montrose DC,Swank DL,Nakanishi M,Ilsley JN,Rosenberg DW||Molecular carcinogenesis (46:60)||2007|
Expression of S100A4 and Met: potential predictors for metastasis and survival in early-stage breast cancer.
MA5-11680 was used in immunohistochemistry to investigate the usefulness of S100A4 and Met gene expression in the prediction of breast cancer status
|Lee WY,Su WC,Lin PW,Guo HR,Chang TW,Chen HH||Oncology (66:429)||2004|
Survivin expression is a prognostic marker in pancreatic cancer patients.
MA5-11680 was used in immunohistochemistry to investigate the usefulness of survivin for pancreatic cancer prognosis
|Kami K,Doi R,Koizumi M,Toyoda E,Mori T,Ito D,Fujimoto K,Wada M,Miyatake S,Imamura M||Surgery (136:443)||2004|
Expression of the inhibitor of apoptosis protein survivin in benign meningiomas.
MA5-11680 was used in immunohistochemistry to investigate the expression of survivin in benign meningiomas
|Das A,Tan WL,Smith DR||Cancer letters (193:217)||2003|
Expression of survivin in primary glioblastomas.
MA5-11680 was used in immunohistochemistry to investigate the survivin expression in primary glioblastomas
|Das A,Tan WL,Teo J,Smith DR||Journal of cancer research and clinical oncology (128:302)||2002|
Ex vivo enrichment of circulating anti-tumor T cells from both cutaneous and ocular melanoma patients: clinical implications for adoptive cell transfer therapy.
MA5-11680 was used in flow cytometry to investigate the efficacy of the adoptive cell transfer protocol for melanoma patients
|Mazzarella T,Cambiaghi V,Rizzo N,Pilla L,Parolini D,Orsenigo E,Colucci A,Modorati G,Doglioni C,Parmiani G,Maccalli C||Cancer immunology, immunotherapy : CII (61:1169)||2012|
|Not Applicable||Not Cited||
Immunobiological characterization of cancer stem cells isolated from glioblastoma patients.
MA5-11680 was used in flow cytometry to characterize glioblastoma multiforme cancer stem cells
|Di Tomaso T,Mazzoleni S,Wang E,Sovena G,Clavenna D,Franzin A,Mortini P,Ferrone S,Doglioni C,Marincola FM,Galli R,Parmiani G,Maccalli C||Clinical cancer research : an official journal of the American Association for Cancer Research (16:800)||2010|
Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis.
MA5-11680 was used in western blot to study the role of SSRP1 in microtubule growth and spindle assembly during mitosis
|Zeng SX,Li Y,Jin Y,Zhang Q,Keller DM,McQuaw CM,Barklis E,Stone S,Hoatlin M,Zhao Y,Lu H||Molecular and cellular biology (30:935)||2010|
p53-dependent antiproliferative and antitumor effect of novel alkyl series of diorganotin(IV) compounds.
MA5-11680 was used in western blot to investigate the effect of diorganotin dichloride complexes on tumor growth
|Koch B,Basu Baul TS,Chatterjee A||Investigational new drugs (27:319)||2009|
Inhibitor of apoptosis protein (IAP) survivin is upregulated by oncogenic c-H-Ras.
MA5-11680 was used in immunocytochemistry and western blot to study the upregulation of survivin by oncogenic c-H-Ras
|Sommer KW,Schamberger CJ,Schmidt GE,Sasgary S,Cerni C||Oncogene (22:4266)||2003|
Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; survivin variant 3 alpha
AP14; API4; BIRC5; EPR-1; IAP4